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To investigate the inhibition of cell proliferation by essential oil of Chenopodium ambrosioides on the human liver cancer SMMC-7721 cells and human liver LO2 cells,and to study the mechanism of anti-tumor in vitro. The inhibition of cell proliferation by essential oil of Chenopodium ambrosioides was determined by MTT assay; the distribution of cell cycle was analyzed by flow cytometry( FCM) with PI staining; cell morphology and apoptosis effect of SMMC-7721 cells were observed by microscope; the apoptotic rate was quantified by FCM with Annexin V / PI double staining. Essential oil of Chenopodium ambrosioides could significantly inhibit the cell proliferation in a concentration-time-dependent manner( P < 0. 05),and the IC50 values on SMMC-7721 cells were lower than human liver LO2 cells at 24,48 and 72 h,respectively( P < 0. 05); cell cycle of SMMC-7721 cells was arrested in G0/G1phase; morphological observation revealed that the cells were wrinkled and the cellular cohesiveness of cells was reduced; nuclear was condensed and in orange colour,of which were the late apoptotic features; and the apoptotic rate increased in a concentration-dependent manner( P < 0. 05),non-viable apoptotic rate was obviously decreased with caspase inhibitor in 100 μg / m L essential oil of Chenopodium ambrosioides( P < 0. 01). Essential oil of Chenopodium ambrosioides can inhibit SMMC-7721 cell proliferation, which may be related to inducing cell cycle arrest and caspase-dependent apoptosis.
Ya-nan Wang, and
Xiao-huan Zhu, and
Hui Ma, and
Run-yuan Du, and
De-rong Li, and
Dan-wei Ma
September 2010,
Pharmaceuticals (Basel, Switzerland),
Ya-nan Wang, and
Xiao-huan Zhu, and
Hui Ma, and
Run-yuan Du, and
De-rong Li, and
Dan-wei Ma
April 2009,
Cancer letters,
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Xiao-huan Zhu, and
Hui Ma, and
Run-yuan Du, and
De-rong Li, and
Dan-wei Ma
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Microbial pathogenesis,
Ya-nan Wang, and
Xiao-huan Zhu, and
Hui Ma, and
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De-rong Li, and
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Chinese journal of integrative medicine,
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Xiao-huan Zhu, and
Hui Ma, and
Run-yuan Du, and
De-rong Li, and
Dan-wei Ma
October 2002,
World journal of gastroenterology,
Ya-nan Wang, and
Xiao-huan Zhu, and
Hui Ma, and
Run-yuan Du, and
De-rong Li, and
Dan-wei Ma
January 2001,
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica,
Ya-nan Wang, and
Xiao-huan Zhu, and
Hui Ma, and
Run-yuan Du, and
De-rong Li, and
Dan-wei Ma
August 2006,
Mitochondrion,
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Hui Ma, and
Run-yuan Du, and
De-rong Li, and
Dan-wei Ma
April 2007,
Journal of economic entomology,
Ya-nan Wang, and
Xiao-huan Zhu, and
Hui Ma, and
Run-yuan Du, and
De-rong Li, and
Dan-wei Ma
January 2006,
Chemotherapy,
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Hui Ma, and
Run-yuan Du, and
De-rong Li, and
Dan-wei Ma
April 2019,
Environmental pollution (Barking, Essex : 1987),
