Biomaterial Database

Symbiotic Potential of Bradyrhizobium japonicum Strains with Different Growth Rates. 2016

D V Krutylo, and N O Leonova

To study genetic properties of soybean nodule bacteria with different growth rates, to research the ability of rhizobia to produce phytohormonal substances and estimate their symbiotic potential in the conditions of combined inoculation and mono-inoculation of soybean plants. Sequencing of the 16S-23S rDNA intergenic spacer of soybean rhizobia was performed on ABI 3130xl Genetic Analyzer. Determination of the qualitative and quantitative composition of phytohormones in culture liquid of Bradyrhizobium japonicum strains was performed by SDTLC-chromatography of high resolution. Symbiotic potential of the strains was estimated in the field experiment in Polissia area of Ukraine. It has been established that strains of soybean nodule bacteria with different growth rates by the structure of ITS region belong to different genetic groups: USDA 6, USDA 110 and USDA 123. The studied rhizobia differ in the levels of extracellular phytohormones synthesis: the slow-growing B. japonicum strain 46 produces more amounts of auxins, but the intensive-growing B. japonicum strain KB11 significantly exceeds in the amounts of cytokinins. It has been shown that an important factor of increasing soybean productivity is the formation of balanced symbiotic systems of the host plant with several complementary strains but serologically and genetically different strains of rhizobia of the same species. Combined inoculation of soybean seeds with two active strains B. japonicum 46 and B. japonicum KB11 allows more fully realizing the potential of symbiotic partners and receiving the higher yield.

UI	MeSH Term	Description	Entries
D004269	DNA, Bacterial	Deoxyribonucleic acid that makes up the genetic material of bacteria.	Bacterial DNA
D012336	RNA, Ribosomal, 16S	Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.	16S Ribosomal RNA,16S rRNA,RNA, 16S Ribosomal,Ribosomal RNA, 16S,rRNA, 16S
D012338	RNA, Ribosomal, 23S	Constituent of 50S subunit of prokaryotic ribosomes containing about 3200 nucleotides. 23S rRNA is involved in the initiation of polypeptide synthesis.	23S Ribosomal RNA,23S rRNA,RNA, 23S Ribosomal,Ribosomal RNA, 23S,rRNA, 23S
D013025	Glycine max	An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.	Soy Beans,Soybeans,Bean, Soy,Beans, Soy,Soy Bean,Soybean
D013559	Symbiosis	The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.	Endosymbiosis,Commensalism,Mutualism
D014455	Ukraine	Country in Europe, bordering the Black Sea, between Poland, Romania, and Moldova in the west and Russia in the east. The capital is Kyiv.
D053204	Root Nodules, Plant	Knobbed structures formed from and attached to plant roots, especially of LEGUMES, which result from symbiotic infection by nitrogen fixing bacteria such as RHIZOBIUM or FRANKIA. Root nodules are structures related to MYCORRHIZAE formed by symbiotic associations with fungi.	Plant Root Nodules,Nodule, Plant Root,Nodules, Plant Root,Plant Root Nodule,Root Nodule, Plant
D059827	Agricultural Inoculants	Beneficial microorganisms (bacteria or fungi) encapsulated in carrier material and applied to the environment for remediation and enhancement of agricultural productivity.	Microbial Inoculants,Bacterial Inoculants,Fungal Inoculants,Agricultural Inoculant,Bacterial Inoculant,Fungal Inoculant,Inoculant, Agricultural,Inoculant, Bacterial,Inoculant, Fungal,Inoculant, Microbial,Inoculants, Agricultural,Inoculants, Bacterial,Inoculants, Fungal,Inoculants, Microbial,Microbial Inoculant
D020369	Bradyrhizobium	A genus of gram-negative, aerobic, rod-shaped bacteria usually containing granules of poly-beta-hydroxybutyrate. They characteristically invade the root hairs of leguminous plants and act as intracellular symbionts.
D021903	DNA, Ribosomal Spacer	The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers).	Ribosomal Spacer DNA,Spacer DNA, Ribosomal