BIOMAT Database Logo BIOMAT Database Logo

Amino acid sequence of mammalian elongation factor 2 deduced from the cDNA sequence: homology with GTP-binding proteins. 1986

K Kohno, and T Uchida, and H Ohkubo, and S Nakanishi, and T Nakanishi, and T Fukui, and E Ohtsuka, and M Ikehara, and Y Okada

Complementary DNA clones, pHEW1 and pRE2, coding for hamster and rat polypeptide chain elongation factor 2 (EF-2), respectively, were isolated and sequenced. It was shown that the cDNA insert in pHEW1 contains a 2574-base-pair open reading frame coding for an 857-amino acid polypeptide with Mr 95,192, excluding the initiation methionine. Comparative studies of sequence homology among EF-2 and several GTP-binding proteins show that five regions in the amino-terminal position of EF-2, corresponding to about 160 amino acids, show homology with GTP-binding proteins, including protein synthesis elongation and initiation factors, mammalian ras proteins, and transducin. The carboxyl-terminal half of EF-2 contains several regions that have 34-75% homology with bacterial elongation factor G. These results suggest that the amino-terminal region of EF-2 participates in the GTP-binding and GTPase activity whereas the carboxyl-terminal region interacts with ribosomes. Finally, the sequence provides direct evidence that diphthamide (2-[3-carboxy-amido-3-(trimethylammonio)propyl]histidine), the site of ADP-ribosylation by diphtheria toxin, is produced by post-translational modification of a histidine residue in the primary translational product.

UI MeSH Term Description Entries
D010445 Peptide Elongation Factors Protein factors uniquely required during the elongation phase of protein synthesis. Elongation Factor,Elongation Factors, Peptide,Factor, Elongation,Factors, Peptide Elongation
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004262 DNA Restriction Enzymes Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D006224 Cricetinae A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS. Cricetus,Hamsters,Hamster
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012689 Sequence Homology, Nucleic Acid The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function. Base Sequence Homology,Homologous Sequences, Nucleic Acid,Homologs, Nucleic Acid Sequence,Homology, Base Sequence,Homology, Nucleic Acid Sequence,Nucleic Acid Sequence Homologs,Nucleic Acid Sequence Homology,Sequence Homology, Base,Base Sequence Homologies,Homologies, Base Sequence,Sequence Homologies, Base
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D019204 GTP-Binding Proteins Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-. G-Proteins,GTP-Regulatory Proteins,Guanine Nucleotide Regulatory Proteins,G-Protein,GTP-Binding Protein,GTP-Regulatory Protein,Guanine Nucleotide Coupling Protein,G Protein,G Proteins,GTP Binding Protein,GTP Binding Proteins,GTP Regulatory Protein,GTP Regulatory Proteins,Protein, GTP-Binding,Protein, GTP-Regulatory,Proteins, GTP-Binding,Proteins, GTP-Regulatory

Related Publications

K Kohno, and T Uchida, and H Ohkubo, and S Nakanishi, and T Nakanishi, and T Fukui, and E Ohtsuka, and M Ikehara, and Y Okada
Primary structure of rat liver elongation factor 2 deduced from the cDNA sequence.
May 1989, FEBS letters,
K Kohno, and T Uchida, and H Ohkubo, and S Nakanishi, and T Nakanishi, and T Fukui, and E Ohtsuka, and M Ikehara, and Y Okada
Amino Acid Sequence of an Algal Peptide Elongation Factor EF-2 Deduced from the Complementary DNA Sequence.
September 1991, Plant physiology,
K Kohno, and T Uchida, and H Ohkubo, and S Nakanishi, and T Nakanishi, and T Fukui, and E Ohtsuka, and M Ikehara, and Y Okada
Octopus rhodopsin. Amino acid sequence deduced from cDNA.
May 1988, FEBS letters,
K Kohno, and T Uchida, and H Ohkubo, and S Nakanishi, and T Nakanishi, and T Fukui, and E Ohtsuka, and M Ikehara, and Y Okada
Amino acid sequence of rat argininosuccinate lyase deduced from cDNA.
January 1988, Journal of biochemistry,
K Kohno, and T Uchida, and H Ohkubo, and S Nakanishi, and T Nakanishi, and T Fukui, and E Ohtsuka, and M Ikehara, and Y Okada
Amino acid sequence of ammodytoxin C as deduced from cDNA.
June 1989, Nucleic acids research,
K Kohno, and T Uchida, and H Ohkubo, and S Nakanishi, and T Nakanishi, and T Fukui, and E Ohtsuka, and M Ikehara, and Y Okada
The cDNA sequence and the deduced amino acid sequence of human transcobalamin II show homology with rat intrinsic factor and human transcobalamin I.
April 1991, The Journal of biological chemistry,
K Kohno, and T Uchida, and H Ohkubo, and S Nakanishi, and T Nakanishi, and T Fukui, and E Ohtsuka, and M Ikehara, and Y Okada
Amino acid sequence of flounder growth hormone deduced from a cDNA sequence.
November 1988, Nucleic acids research,
K Kohno, and T Uchida, and H Ohkubo, and S Nakanishi, and T Nakanishi, and T Fukui, and E Ohtsuka, and M Ikehara, and Y Okada
Mouse ornithine decarboxylase. Complete amino acid sequence deduced from cDNA.
March 1985, The Journal of biological chemistry,
K Kohno, and T Uchida, and H Ohkubo, and S Nakanishi, and T Nakanishi, and T Fukui, and E Ohtsuka, and M Ikehara, and Y Okada
Amino acid sequence of a muscarinic toxin deduced from the cDNA nucleotide sequence.
January 1991, Toxicon : official journal of the International Society on Toxinology,
K Kohno, and T Uchida, and H Ohkubo, and S Nakanishi, and T Nakanishi, and T Fukui, and E Ohtsuka, and M Ikehara, and Y Okada
Amino acid sequence of silkworm (Bombyx mori) hemolymph antitrypsin deduced from its cDNA nucleotide sequence: confirmation of its homology with serpins.
September 1990, Journal of biochemistry,
Copied contents to your clipboard!