OBJECTIVE The aim of this research was to identify the seroprevalence of Neospora caninum in local Bali dogs. METHODS A total of 147 local Bali dog serum samples have been examined for antibodies of N. caninum using enzyme-linked immunosorbent assay test. RESULTS The results confirmed that 5 (3.4%) local Bali dogs have antibody for N. caninum. There were no significant differences in seroprevalence of Neospora infection in local Bali dogs between different genders, ages, and take care methods (p>0.05). CONCLUSIONS The results provided evidence for the presence of N. caninum infection in local Bali dogs and thus the risk to Bali cattle and dog health.