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Two mouse monoclonal antibodies, NE-25 and PE-35, defining novel cell surface antigens of small cell lung carcinoma (SCLC) were produced. The molecular weight of NE-25 and PE-35 antigens estimated by radioimmunoprecipitation was 25,000 and 35,000, respectively. NE-25 antigen was expressed on the majority of cell lines and tumor specimens of SCLC among lung carcinoma. These NE-25-positive cell lines showed typical growth morphology as SCLC classic lines and expressed high levels of neuroendocrine biomarkers, such as aromatic L-amino acid decarboxylase, while NE-25 antigen-negative lines lacked apparent neuroendocrine properties. This antigen was expressed also on a subset of neoplastic cells with (neuro)endocrine properties, including pulmonary carcinoid, and on various tumors of nervous tissues, such as neuroblastoma. Among the normal cells, Kulchitski cells of lung, thyroid gland, adrenal gland, Langerhans islet, and nervous tissues were positive. Thus, the expression of NE-25 antigen is closely associated with the neural and/or (neuro)endocrine differentiation state. On the contrary, PE-35 antigen was present on four major types of lung carcinomas as well as on squamous cell carcinoma and adenocarcinomas of various tissues, but it was absent from nervous tissue tumors. Thus, PE-35 antibody showed a "pan-epithelial" reactivity. Analysis by NE-25 and PE-35 antibodies provided evidence for the heterogeneities of SCLC by demonstrating four surface phenotypes, with the NE-25+/PE-35+ phenotype being most common. In addition, the results supported the current understanding that various histological types of lung carcinoma, including SCLC, are derived from a stem cell of the bronchial epithelium.
T Takahashi, and
R Ueda, and
X Song, and
K Nishida, and
M Shinzato, and
R Namikawa, and
Y Ariyoshi, and
K Ota, and
K Kato, and
T Nagatsu
January 1984,
Proceedings of the National Academy of Sciences of the United States of America,
T Takahashi, and
R Ueda, and
X Song, and
K Nishida, and
M Shinzato, and
R Namikawa, and
Y Ariyoshi, and
K Ota, and
K Kato, and
T Nagatsu
June 1983,
Journal of immunology (Baltimore, Md. : 1950),
T Takahashi, and
R Ueda, and
X Song, and
K Nishida, and
M Shinzato, and
R Namikawa, and
Y Ariyoshi, and
K Ota, and
K Kato, and
T Nagatsu
January 1984,
Proceedings of the National Academy of Sciences of the United States of America,
T Takahashi, and
R Ueda, and
X Song, and
K Nishida, and
M Shinzato, and
R Namikawa, and
Y Ariyoshi, and
K Ota, and
K Kato, and
T Nagatsu
August 1981,
Proceedings of the National Academy of Sciences of the United States of America,
T Takahashi, and
R Ueda, and
X Song, and
K Nishida, and
M Shinzato, and
R Namikawa, and
Y Ariyoshi, and
K Ota, and
K Kato, and
T Nagatsu
November 1984,
Cancer research,
T Takahashi, and
R Ueda, and
X Song, and
K Nishida, and
M Shinzato, and
R Namikawa, and
Y Ariyoshi, and
K Ota, and
K Kato, and
T Nagatsu
June 1986,
Cancer research,
T Takahashi, and
R Ueda, and
X Song, and
K Nishida, and
M Shinzato, and
R Namikawa, and
Y Ariyoshi, and
K Ota, and
K Kato, and
T Nagatsu
November 1983,
European journal of immunology,
T Takahashi, and
R Ueda, and
X Song, and
K Nishida, and
M Shinzato, and
R Namikawa, and
Y Ariyoshi, and
K Ota, and
K Kato, and
T Nagatsu
July 1983,
[Hokkaido igaku zasshi] The Hokkaido journal of medical science,
T Takahashi, and
R Ueda, and
X Song, and
K Nishida, and
M Shinzato, and
R Namikawa, and
Y Ariyoshi, and
K Ota, and
K Kato, and
T Nagatsu
September 1982,
Proceedings of the National Academy of Sciences of the United States of America,
T Takahashi, and
R Ueda, and
X Song, and
K Nishida, and
M Shinzato, and
R Namikawa, and
Y Ariyoshi, and
K Ota, and
K Kato, and
T Nagatsu
May 2002,
Current protocols in immunology,
