-transparent.png){kind=logo}
We used immunofluorescence to examine the developmental relationship of Ia and IgD on B cells. Pre-B cells in fetal liver did not express Ia. Only very few surface IgM-positive (sIgM+) B cells in fetal spleen were found to be Ia+ and were weakly stained for Ia. After birth there was a linear increase in the proportion of sIgM+ spleen cells which expressed Ia, reaching 95% by 9 days. Adult bone marrow also contains a sizeable proportion of sIgM+ Ia- cells. Unstimulated cells from fetal or newborn liver and spleen expressed Ia at the same rate in culture. Anti-Ia antisera suppressed the LPS-induced differentiation of IgM and IgG plasma cells in cultures of neonatal lymphocytes. Ia was also detected on IgM and IgG plasma cells in vitro suggesting that lipopolysaccharide (LPS)-stimulated B cells by may express Ia antigens, induced by LPS, or appearing as part of normal differentiation. IgD did not appear on sIgM+ cells until 3 days of age and then rose slowly to reach adult levels later than Ia antigens.
J F Kearney, and
M D Cooper, and
J Klein, and
E R Abney, and
R M Parkhouse, and
A R Lawton
January 1982,
Annals of the New York Academy of Sciences,
J F Kearney, and
M D Cooper, and
J Klein, and
E R Abney, and
R M Parkhouse, and
A R Lawton
April 1986,
The Journal of experimental medicine,
J F Kearney, and
M D Cooper, and
J Klein, and
E R Abney, and
R M Parkhouse, and
A R Lawton
December 1993,
Journal of immunological methods,
J F Kearney, and
M D Cooper, and
J Klein, and
E R Abney, and
R M Parkhouse, and
A R Lawton
January 1982,
Annals of the New York Academy of Sciences,
J F Kearney, and
M D Cooper, and
J Klein, and
E R Abney, and
R M Parkhouse, and
A R Lawton
March 1982,
Molecular immunology,
J F Kearney, and
M D Cooper, and
J Klein, and
E R Abney, and
R M Parkhouse, and
A R Lawton
December 1977,
Immunology,
J F Kearney, and
M D Cooper, and
J Klein, and
E R Abney, and
R M Parkhouse, and
A R Lawton
November 1979,
European journal of immunology,
J F Kearney, and
M D Cooper, and
J Klein, and
E R Abney, and
R M Parkhouse, and
A R Lawton
January 1979,
Monographs in allergy,
J F Kearney, and
M D Cooper, and
J Klein, and
E R Abney, and
R M Parkhouse, and
A R Lawton
February 1992,
International immunology,
J F Kearney, and
M D Cooper, and
J Klein, and
E R Abney, and
R M Parkhouse, and
A R Lawton
November 1975,
Journal of immunology (Baltimore, Md. : 1950),
