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We examined the usefulness of a counterimmunoelectrophoresis (CIE) technique for detecting antibodies to HTLV-III using sera that previously had been assessed for antibodies to HTLV-III by the standard enzyme-linked immunosorbent assay (ELISA). We selected a subset of 53 sera from patients with the acquired immune deficiency syndrome (AIDS) or the generalized lymphadenopathy syndrome (GLS) in which 81.1% were initially ELISA-positive, and 96.2% were positive by Western blot technique. In our standard HTLV-III CIE technique, 58.5% were positive and repeat testing increased the yield to 67.9%. Varying several parameters of the standard CIE assay did not improve sensitivity. We also studied 20 ELISA-negative and 10 ELISA-borderline sera from normal controls; all were negative by CIE. These results indicate that CIE may be used for detection of human serum antibodies to HTLV-III, but that the present assay was less sensitive than the HTLV-III ELISA.
M J Blaser, and
D L Cohn, and
H J Cody, and
K A Penley, and
F N Judson, and
W C Saxinger, and
S H Weiss
July 1988,
Rinsho byori. The Japanese journal of clinical pathology,
M J Blaser, and
D L Cohn, and
H J Cody, and
K A Penley, and
F N Judson, and
W C Saxinger, and
S H Weiss
December 1978,
The Journal of pediatrics,
M J Blaser, and
D L Cohn, and
H J Cody, and
K A Penley, and
F N Judson, and
W C Saxinger, and
S H Weiss
September 1985,
CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne,
M J Blaser, and
D L Cohn, and
H J Cody, and
K A Penley, and
F N Judson, and
W C Saxinger, and
S H Weiss
September 1986,
Medizinische Monatsschrift fur Pharmazeuten,
M J Blaser, and
D L Cohn, and
H J Cody, and
K A Penley, and
F N Judson, and
W C Saxinger, and
S H Weiss
October 1985,
The New England journal of medicine,
M J Blaser, and
D L Cohn, and
H J Cody, and
K A Penley, and
F N Judson, and
W C Saxinger, and
S H Weiss
August 1979,
Journal of clinical microbiology,
M J Blaser, and
D L Cohn, and
H J Cody, and
K A Penley, and
F N Judson, and
W C Saxinger, and
S H Weiss
July 1986,
The Alabama journal of medical sciences,
M J Blaser, and
D L Cohn, and
H J Cody, and
K A Penley, and
F N Judson, and
W C Saxinger, and
S H Weiss
December 1986,
AIDS research,
M J Blaser, and
D L Cohn, and
H J Cody, and
K A Penley, and
F N Judson, and
W C Saxinger, and
S H Weiss
November 1986,
JAMA,
M J Blaser, and
D L Cohn, and
H J Cody, and
K A Penley, and
F N Judson, and
W C Saxinger, and
S H Weiss
June 1983,
The Indian journal of medical research,
