-transparent.png){kind=logo}
A cDNA library was constructed from a DR7, DRw53, DQw2 homozygous cell line, cDNA clones corresponding to DR beta and DQ beta chains were isolated, and the nucleotide sequences of the polymorphic first domains of these chains were determined. A novel screening strategy allowed rapid and simple identification of cDNA clones corresponding to both DR beta chains (DR7 beta1 and DR7 beta2): DR7 beta2 clones have a recognition site for the enzyme BssHII, whereas DR7 beta1 clones do not. The DR7 beta 1 sequence differs significantly from all previously described DR beta chains. As predicted by the presence of the BssHII site in DR7 beta 2 clones, the DR7 beta 2 sequence differs from the DR7 beta 1 sequence. The sequence of the DRw53-associated DR7 beta 2 chain is identical to the reported sequence of the DRw53-associated DR4 beta 2 chain. In addition, the sequence of the DQ beta chain from the DR7, DQw2 cell line is identical to the reported sequence of a DQ beta chain from a DR3, DQw2 cell. These findings raise interesting questions about the evolution of the DR3, DR4, and DR7 haplotypes.
R W Karr, and
P K Gregersen, and
F Obata, and
D Goldberg, and
J Maccari, and
C Alber, and
J Silver
July 1988,
Human immunology,
R W Karr, and
P K Gregersen, and
F Obata, and
D Goldberg, and
J Maccari, and
C Alber, and
J Silver
February 1986,
The Journal of biological chemistry,
R W Karr, and
P K Gregersen, and
F Obata, and
D Goldberg, and
J Maccari, and
C Alber, and
J Silver
September 1987,
Tissue antigens,
R W Karr, and
P K Gregersen, and
F Obata, and
D Goldberg, and
J Maccari, and
C Alber, and
J Silver
September 1987,
Tissue antigens,
R W Karr, and
P K Gregersen, and
F Obata, and
D Goldberg, and
J Maccari, and
C Alber, and
J Silver
September 1989,
Journal of immunology (Baltimore, Md. : 1950),
R W Karr, and
P K Gregersen, and
F Obata, and
D Goldberg, and
J Maccari, and
C Alber, and
J Silver
January 1989,
Immunogenetics,
R W Karr, and
P K Gregersen, and
F Obata, and
D Goldberg, and
J Maccari, and
C Alber, and
J Silver
February 1992,
Transplantation proceedings,
R W Karr, and
P K Gregersen, and
F Obata, and
D Goldberg, and
J Maccari, and
C Alber, and
J Silver
February 1987,
Transplantation proceedings,
R W Karr, and
P K Gregersen, and
F Obata, and
D Goldberg, and
J Maccari, and
C Alber, and
J Silver
January 1983,
The EMBO journal,
R W Karr, and
P K Gregersen, and
F Obata, and
D Goldberg, and
J Maccari, and
C Alber, and
J Silver
January 1982,
Proceedings of the National Academy of Sciences of the United States of America,
