Bioengineering functional smooth muscle with spontaneous rhythmic contraction in vitro. 2018

Masae Kobayashi, and Hassan A Khalil, and Nan Ye Lei, and Qianqian Wang, and Ke Wang, and Benjamin M Wu, and James C Y Dunn
Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.

Oriented smooth muscle layers in the intestine contract rhythmically due to the action of interstitial cells of Cajal (ICC) that serve as pacemakers of the intestine. Disruption of ICC networks has been reported in various intestinal motility disorders, which limit the quality and expectancy of life. A significant challenge in intestinal smooth muscle engineering is the rapid loss of function in cultured ICC and smooth muscle cells (SMC). Here we demonstrate a novel approach to maintain the function of both ICC and SMC in vitro. Primary intestinal SMC mixtures cultured on feeder cells seeded electrospun poly(3-caprolactone) scaffolds exhibited rhythmic contractions with directionality for over 10 weeks in vitro. The simplicity of this system should allow for wide usage in research on intestinal motility disorders and tissue engineering, and may prove to be a versatile platform for generating other types of functional SMC in vitro.

UI MeSH Term Description Entries
D007410 Intestinal Diseases Pathological processes in any segment of the INTESTINE from DUODENUM to RECTUM. Disease, Intestinal,Diseases, Intestinal,Intestinal Disease
D007422 Intestines The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE. Intestine
D008297 Male Males
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D009119 Muscle Contraction A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments. Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009130 Muscle, Smooth Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed) Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D011091 Polyesters Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours. Polyester
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003113 Colonoscopy Endoscopic examination, therapy or surgery of the luminal surface of the colon. Colonoscopic Surgical Procedures,Surgical Procedures, Colonoscopic,Colonoscopic Surgery,Surgery, Colonoscopic,Colonoscopic Surgeries,Colonoscopic Surgical Procedure,Colonoscopies,Procedure, Colonoscopic Surgical,Procedures, Colonoscopic Surgical,Surgeries, Colonoscopic,Surgical Procedure, Colonoscopic
D005260 Female Females

Related Publications

Masae Kobayashi, and Hassan A Khalil, and Nan Ye Lei, and Qianqian Wang, and Ke Wang, and Benjamin M Wu, and James C Y Dunn
July 2008, Biotechnology and applied biochemistry,
Masae Kobayashi, and Hassan A Khalil, and Nan Ye Lei, and Qianqian Wang, and Ke Wang, and Benjamin M Wu, and James C Y Dunn
October 1990, Canadian journal of physiology and pharmacology,
Masae Kobayashi, and Hassan A Khalil, and Nan Ye Lei, and Qianqian Wang, and Ke Wang, and Benjamin M Wu, and James C Y Dunn
December 1964, Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke,
Masae Kobayashi, and Hassan A Khalil, and Nan Ye Lei, and Qianqian Wang, and Ke Wang, and Benjamin M Wu, and James C Y Dunn
March 1970, Science (New York, N.Y.),
Masae Kobayashi, and Hassan A Khalil, and Nan Ye Lei, and Qianqian Wang, and Ke Wang, and Benjamin M Wu, and James C Y Dunn
November 2013, Physiological reports,
Masae Kobayashi, and Hassan A Khalil, and Nan Ye Lei, and Qianqian Wang, and Ke Wang, and Benjamin M Wu, and James C Y Dunn
April 2011, The Canadian journal of urology,
Masae Kobayashi, and Hassan A Khalil, and Nan Ye Lei, and Qianqian Wang, and Ke Wang, and Benjamin M Wu, and James C Y Dunn
October 1984, Circulation research,
Masae Kobayashi, and Hassan A Khalil, and Nan Ye Lei, and Qianqian Wang, and Ke Wang, and Benjamin M Wu, and James C Y Dunn
September 1980, Biochemical and biophysical research communications,
Masae Kobayashi, and Hassan A Khalil, and Nan Ye Lei, and Qianqian Wang, and Ke Wang, and Benjamin M Wu, and James C Y Dunn
December 1989, Nihon Heikatsukin Gakkai zasshi,
Masae Kobayashi, and Hassan A Khalil, and Nan Ye Lei, and Qianqian Wang, and Ke Wang, and Benjamin M Wu, and James C Y Dunn
July 1977, The American journal of physiology,
Copied contents to your clipboard!