BIOMAT Database Logo BIOMAT Database Logo

[Inhibition of proliferation of adult T-cell leukemia cells by celastrol]. 2018

Jinyong Fang, and Kaining Yi, and Zaowen Song, and Mengyun Chen, and Lingling Xu, and Tiejun Zhao
College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.

We studied the effect of celastrol on the proliferation and apoptosis of adult T-cell leukemia (ATL) cells. After treating adult T-cell leukemia cell lines with different concentrations of celastrol, we analyzed the cell proliferation by MTT and colony formation assays. Flow cytometry was conducted to detect cell apoptosis by Annexin V-FITC/PI staining. Western blotting and dual-luciferase reporter assay were done to study the mechanism how celastrol suppressed the growth of adult T-cell leukemia cells. Celastrol could significantly inhibit the proliferation of adult T-cell leukemia cells, and induce apoptosis of ATL cells. With the increase of the concentration of celastrol, the ratio of Bax/Bcl-2 protein was up-regulated. The Caspase-3/7 protein was cleaved and activated after treatment with celastrol. Moreover, the expression of HTLV-1-encoded viral protein Tax was significantly inhibited in the celastrol treated cells. Taken together, these results indicated that celastrol effectively inhibited the proliferation of adult T-cell leukemia cells by regulating the expression of Bcl-2 family protein, and induced cell apoptosis by activating Caspase dependent pathway. In addition, celastrol could inhibit the expression of viral protein Tax. This study will provide an experimental basis for the clinical application of celastrol in the treatment of adult T-cell leukemia.

UI MeSH Term Description Entries
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014315 Triterpenes A class of terpenes (the general formula C30H48) formed by the condensation of six isoprene units, equivalent to three terpene units. Triterpene,Triterpenoid,Triterpenoids
D015459 Leukemia-Lymphoma, Adult T-Cell Aggressive T-Cell malignancy with adult onset, caused by HUMAN T-LYMPHOTROPIC VIRUS 1. It is endemic in Japan, the Caribbean basin, Southeastern United States, Hawaii, and parts of Central and South America and sub-Saharan Africa. ATLL,HTLV I Associated T Cell Leukemia Lymphoma,HTLV-Associated Leukemia-Lymphoma,HTLV-I-Associated T-Cell Leukemia-Lymphoma,Human T Lymphotropic Virus Associated Leukemia Lymphoma,Human T Lymphotropic Virus-Associated Leukemia-Lymphoma,Human T-Cell Leukemia-Lymphoma,Leukemia Lymphoma, Adult T Cell,Leukemia Lymphoma, T Cell, Acute, HTLV I Associated,Leukemia, Adult T-Cell,Leukemia-Lymphoma, T-Cell, Acute, HTLV-I-Associated,T Cell Leukemia Lymphoma, HTLV I Associated,T Cell Leukemia, Adult,T-Cell Leukemia, Adult,T-Cell Leukemia-Lymphoma, Adult,T-Cell Leukemia-Lymphoma, HTLV-I-Associated,Adult T-Cell Leukemia,Adult T-Cell Leukemia-Lymphoma,Adult T-Cell Leukemia-Lymphomas,Adult T-Cell Leukemias,HTLV Associated Leukemia Lymphoma,HTLV-Associated Leukemia-Lymphomas,HTLV-I-Associated T-Cell Leukemia-Lymphomas,Human T Cell Leukemia Lymphoma,Human T-Cell Leukemia-Lymphomas,Leukemia, Adult T Cell,Leukemia-Lymphoma, HTLV-Associated,Leukemia-Lymphoma, HTLV-I-Associated T-Cell,Leukemia-Lymphoma, Human T-Cell,Leukemia-Lymphomas, Adult T-Cell,Leukemia-Lymphomas, HTLV-Associated,Leukemia-Lymphomas, HTLV-I-Associated T-Cell,Leukemia-Lymphomas, Human T-Cell,Leukemias, Adult T-Cell,T Cell Leukemia Lymphoma, Adult,T-Cell Leukemia-Lymphoma, Human,T-Cell Leukemia-Lymphomas, Adult,T-Cell Leukemia-Lymphomas, HTLV-I-Associated,T-Cell Leukemia-Lymphomas, Human,T-Cell Leukemias, Adult
D016356 Gene Products, tax Transcriptional trans-acting proteins of the promoter elements found in the long terminal repeats (LTR) of HUMAN T-LYMPHOTROPIC VIRUS 1 and HUMAN T-LYMPHOTROPIC VIRUS 2. The tax (trans-activator x; x is undefined) proteins act by binding to enhancer elements in the LTR. Trans-Activator Protein p40(tax),Trans-Activator Protein p40(x),p40(tax),tax Gene Products,tax Protein,Gene Product, tax,Trans-Activator Protein p40(lor),Trans-Activator Protein p40x,Trans-Activator Protein pX,Transactivator Protein p40(x),Transactivator p40(tax),Transforming Antigen p40x,p40 tax,Antigen p40x, Transforming,Trans Activator Protein p40x,Trans Activator Protein pX,p40x, Trans-Activator Protein,p40x, Transforming Antigen,pX, Trans-Activator Protein,tax Gene Product
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D049109 Cell Proliferation All of the processes involved in increasing CELL NUMBER including CELL DIVISION. Cell Growth in Number,Cellular Proliferation,Cell Multiplication,Cell Number Growth,Growth, Cell Number,Multiplication, Cell,Number Growth, Cell,Proliferation, Cell,Proliferation, Cellular
D053978 Pentacyclic Triterpenes Five-ring derivatives of dammarane having a chair-chair-chair-boat configuration. They include the lupanes, oleananes, amyrins, GLYCYRRHIZIC ACID, and soyasaponins. Adianane Derivatives,Adiananes,Baccharane Triterpenes,Fernane Derivatives,Fernanes,Gammacerane Derivatives,Gammaceranes,Hopane Derivatives,Hopanes,Hopenoid Derivatives,Pentacyclic Triterpenoids,Triterpenes, Pentacyclic,Triterpenes, Baccharane

Related Publications

Jinyong Fang, and Kaining Yi, and Zaowen Song, and Mengyun Chen, and Lingling Xu, and Tiejun Zhao
[Inhibition of proliferation by retinoic acid on adult T cell leukemia cells].
October 1996, Nihon Rinsho Men'eki Gakkai kaishi = Japanese journal of clinical immunology,
Jinyong Fang, and Kaining Yi, and Zaowen Song, and Mengyun Chen, and Lingling Xu, and Tiejun Zhao
Inhibition of proliferation and CD25 down-regulation by retinoic acid in human adult T cell leukemia cells.
March 1997, Leukemia,
Jinyong Fang, and Kaining Yi, and Zaowen Song, and Mengyun Chen, and Lingling Xu, and Tiejun Zhao
[Mechanism of cell proliferation of adult T cell leukemia (ATL) cells].
May 1996, [Rinsho ketsueki] The Japanese journal of clinical hematology,
Jinyong Fang, and Kaining Yi, and Zaowen Song, and Mengyun Chen, and Lingling Xu, and Tiejun Zhao
Interleukin-4 induces proliferation of adult T-cell leukemia cells.
March 1993, European journal of haematology,
Jinyong Fang, and Kaining Yi, and Zaowen Song, and Mengyun Chen, and Lingling Xu, and Tiejun Zhao
Inhibition of adult T-cell leukemia cell proliferation by polymerized proanthocyanidin from blueberry leaves through JAK proteolysis.
April 2022, Cancer science,
Jinyong Fang, and Kaining Yi, and Zaowen Song, and Mengyun Chen, and Lingling Xu, and Tiejun Zhao
Correlation of aberrant proliferation with T-cell growth factor in adult T-cell leukemia cells.
January 1983, Hematological oncology,
Jinyong Fang, and Kaining Yi, and Zaowen Song, and Mengyun Chen, and Lingling Xu, and Tiejun Zhao
mTOR inhibition and adult T-cell leukemia.
April 2009, Leukemia & lymphoma,
Jinyong Fang, and Kaining Yi, and Zaowen Song, and Mengyun Chen, and Lingling Xu, and Tiejun Zhao
Activation of Notch1 signaling by HTLV-1 Tax promotes proliferation of adult T-cell leukemia cells.
May 2019, Biochemical and biophysical research communications,
Jinyong Fang, and Kaining Yi, and Zaowen Song, and Mengyun Chen, and Lingling Xu, and Tiejun Zhao
Organosilicon compounds as adult T-cell leukemia cell proliferation inhibitors.
January 2013, Chemical & pharmaceutical bulletin,
Jinyong Fang, and Kaining Yi, and Zaowen Song, and Mengyun Chen, and Lingling Xu, and Tiejun Zhao
Inhibition of proliferation and induction of apoptosis by 2-benzoyl-3-phenyl-6,7-dichloroquinoxaline 1,4-dioxide in adult T-cell leukemia cells.
July 2004, Chemico-biological interactions,
Copied contents to your clipboard!