Isolation of lytic bacteriophages infecting Salmonella Typhimurium and Salmonella Enteritidis. 2018

Zeliha Yildirim, and Tuba Sakіn, and Fatma Çoban
Niğde Ömer Halisdemir University , Faculty of Engineering, Department of Food Engineering, Niğde , Turkey.

The objectives of this study were to isolate, purify and determine host range of lytic bacteriophages infecting foodborne the pathogen Salmonella Typhimurium and S. Enteritidis. River/stream water, sewage, raw foods, wastewater from food processing plants, slaughterhouse and fish farms and water from troughs were used for the screening of bacteriophages. The richest sources in terms of phages infecting S. Typhimurium and Enteritidis were found to be sewage, wastewaters of slaughterhouse, food processing and fisheries and streams. A total of 33 S. Typhimurium and 56 S. Enteritidis phages were isolated and purified from the samples. It was demostrated that host ranges of the isolated phages were quite wide. The numbers of bacteria types inhibited by S. Typhimurium or Enteritidis phages were changed among 1-15 and 1-19, respectively. It was found that 75.8% (25 out of 33) and 83.93% (47 out of 56) of isolated S. Typhimurium or Enteritidis phages formed clear plaques and were capable of lysing at least six or two Salmonella serovars. Beside Salmonella serovars, some S. Typhimurium (15 out of 33, 45.5%) and S. Enteritidis phages (5 out of 56, 8.93%) were also infective against E. coli strains. The host ranges of S. Typhimurium phages were wider than those of S. Enteritidis.

UI MeSH Term Description Entries
D012477 Salmonella enteritidis A serotype of Salmonella enterica which is an etiologic agent of gastroenteritis in man and other animals. Salmonella enterica serovar enteritidis
D012483 Salmonella Phages Viruses whose host is Salmonella. A frequently encountered Salmonella phage is BACTERIOPHAGE P22. Salmonella Bacteriophages,Bacteriophage, Salmonella,Bacteriophages, Salmonella,Salmonella Bacteriophage,Salmonella Phage
D012486 Salmonella typhimurium A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER. Salmonella typhimurium LT2
D058507 Host Specificity The properties of a pathogen that makes it capable of infecting one or more specific hosts. The pathogen can include PARASITES as well as VIRUSES; BACTERIA; FUNGI; or PLANTS. Host Specialization,Host Range,Host Species Specificity,Host Ranges,Host Specializations,Host Species Specificities,Host Specificities,Range, Host,Ranges, Host,Specialization, Host,Specializations, Host,Specificities, Host,Specificities, Host Species,Specificity, Host,Specificity, Host Species

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