-transparent.png){kind=logo}
125I-VIP bound specifically to sites on human, rat, guinea pig, and rabbit lung membranes with a dissociation constant (KD) of 60-200 pM and binding site maxima of 200-800 fmol/mg of protein. The presence of a second lower affinity site was detected but not investigated further. High affinity 125I-VIP binding was reversible and displaced by structurally related peptides with an order of potency: VIP greater than rGRF greater than PHI greater than hGRF greater than secretin = Ac Tyr1 D Phe2 GRF. 125I-VIP has been covalently incorporated into lung membranes using disuccinimidyl suberate. Sodium dodecyl sulfate-polyacrilamide gel electrophoresis of labeled human, rat, and rabbit lung membranes revealed major 125I-VIP-receptor complexes of: Mr = 65,000, 56,000, and 64,000 daltons, respectively. Guinea pig lung membranes exhibited two 125I-VIP-receptor complexes of Mr = 66,000 and 60,000 daltons. This labeling pattern probably reflects the presence of differentially glycosylated forms of the same receptor since treatment with neuroaminidase resulted in a single homogeneous band (Mr = 57,000 daltons). Soluble covalently labeled VIP receptors from guinea pig and human lung bound to and were specifically eluted from agarose-linked wheat germ agglutinin columns. Our studies indicate that mammalian lung VIP receptors are glycoproteins containing terminal sialic acid residues.
K E Dickinson, and
M Schachter, and
C M Miles, and
D H Coy, and
P S Sever
January 1978,
Journal of clinical pathology. Supplement (Association of Clinical Pathologists),
K E Dickinson, and
M Schachter, and
C M Miles, and
D H Coy, and
P S Sever
April 1991,
Regulatory peptides,
K E Dickinson, and
M Schachter, and
C M Miles, and
D H Coy, and
P S Sever
August 1987,
Biochimica et biophysica acta,
K E Dickinson, and
M Schachter, and
C M Miles, and
D H Coy, and
P S Sever
September 2000,
Peptides,
K E Dickinson, and
M Schachter, and
C M Miles, and
D H Coy, and
P S Sever
December 1979,
FEBS letters,
K E Dickinson, and
M Schachter, and
C M Miles, and
D H Coy, and
P S Sever
January 1988,
Peptides,
K E Dickinson, and
M Schachter, and
C M Miles, and
D H Coy, and
P S Sever
January 2000,
Immunology today,
K E Dickinson, and
M Schachter, and
C M Miles, and
D H Coy, and
P S Sever
October 2011,
Peptides,
K E Dickinson, and
M Schachter, and
C M Miles, and
D H Coy, and
P S Sever
October 1978,
Archives internationales de physiologie et de biochimie,
K E Dickinson, and
M Schachter, and
C M Miles, and
D H Coy, and
P S Sever
January 1987,
The Journal of biological chemistry,
