N6 -Methyladenosine-Sensitive RNA-Cleaving Deoxyribozymes. 2018

Maksim V Sednev, and Volodymyr Mykhailiuk, and Priyanka Choudhury, and Julia Halang, and Katherine E Sloan, and Markus T Bohnsack, and Claudia Höbartner
Universität Würzburg, Institut für Organische Chemie, Am Hubland, 97074, Würzburg, Germany.

Deoxyribozymes are synthetic enzymes made of DNA that can catalyze the cleavage or formation of phosphodiester bonds and are useful tools for RNA biochemistry. Herein, we report new RNA-cleaving deoxyribozymes to interrogate the methylation status of target RNAs, thereby providing an alternative method for the biochemical validation of RNA methylation sites containing N6 -methyladenosine, which is the most wide-spread and extensively investigated natural RNA modification. The developed deoxyribozymes are sensitive to the presence of N6 -methyladenosine in RNA near the cleavage site. One class of these DNA enzymes shows faster cleavage of methylated RNA, while others are strongly inhibited by the modified nucleotide. The general applicability of the new deoxyribozymes is demonstrated for several examples of natural RNA sequences, including a lncRNA and a set of C/D box snoRNAs, which have been suggested to contain m6 A as a regulatory element that influences RNA folding and protein binding.

UI MeSH Term Description Entries
D008745 Methylation Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed) Methylations
D009690 Nucleic Acid Conformation The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape. DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D000241 Adenosine A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter. Adenocard,Adenoscan
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012313 RNA A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed) RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D059367 RNA Cleavage A reaction that severs one of the sugar-phosphate linkages of the phosphodiester backbone of RNA. It is catalyzed enzymatically, chemically, or by radiation. Cleavage may be exonucleolytic, or endonucleolytic. Cleavage, RNA,Cleavages, RNA,RNA Cleavages
D021881 DNA, Catalytic Molecules of DNA that possess enzymatic activity. Catalytic DNA,Deoxyribozymes,DNAzyme,DNAzymes,Deoxyribozyme

Related Publications

Maksim V Sednev, and Volodymyr Mykhailiuk, and Priyanka Choudhury, and Julia Halang, and Katherine E Sloan, and Markus T Bohnsack, and Claudia Höbartner
August 2021, Angewandte Chemie (International ed. in English),
Maksim V Sednev, and Volodymyr Mykhailiuk, and Priyanka Choudhury, and Julia Halang, and Katherine E Sloan, and Markus T Bohnsack, and Claudia Höbartner
April 2016, Yi chuan = Hereditas,
Maksim V Sednev, and Volodymyr Mykhailiuk, and Priyanka Choudhury, and Julia Halang, and Katherine E Sloan, and Markus T Bohnsack, and Claudia Höbartner
January 2015, PloS one,
Maksim V Sednev, and Volodymyr Mykhailiuk, and Priyanka Choudhury, and Julia Halang, and Katherine E Sloan, and Markus T Bohnsack, and Claudia Höbartner
August 2003, RNA (New York, N.Y.),
Maksim V Sednev, and Volodymyr Mykhailiuk, and Priyanka Choudhury, and Julia Halang, and Katherine E Sloan, and Markus T Bohnsack, and Claudia Höbartner
January 2022, Frontiers in cardiovascular medicine,
Maksim V Sednev, and Volodymyr Mykhailiuk, and Priyanka Choudhury, and Julia Halang, and Katherine E Sloan, and Markus T Bohnsack, and Claudia Höbartner
October 2019, Molecular cell,
Maksim V Sednev, and Volodymyr Mykhailiuk, and Priyanka Choudhury, and Julia Halang, and Katherine E Sloan, and Markus T Bohnsack, and Claudia Höbartner
January 2019, Wiley interdisciplinary reviews. RNA,
Maksim V Sednev, and Volodymyr Mykhailiuk, and Priyanka Choudhury, and Julia Halang, and Katherine E Sloan, and Markus T Bohnsack, and Claudia Höbartner
December 2013, Yi chuan = Hereditas,
Maksim V Sednev, and Volodymyr Mykhailiuk, and Priyanka Choudhury, and Julia Halang, and Katherine E Sloan, and Markus T Bohnsack, and Claudia Höbartner
December 2023, Autoimmunity,
Maksim V Sednev, and Volodymyr Mykhailiuk, and Priyanka Choudhury, and Julia Halang, and Katherine E Sloan, and Markus T Bohnsack, and Claudia Höbartner
April 2022, Nature communications,
Copied contents to your clipboard!