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Intracellular pH (pHi) was measured directly in isolated rat hepatocyte couplets using pH sensitive microelectrodes. The hepatocytes were maintained in a minimal salt buffer without added hormones or serum. Values of pHi (6.99 +/- 0.12, mean +/- SE) were close to their Nernst equilibria. After intracellular acidification with ammonium chloride, pH regulation was inhibited with 1 mM amiloride or by omission of external sodium, consistent with a Na-H exchange mechanism. Mean intracellular buffering power, in the nominal absence of carbon dioxide, was 34.1 +/- 11.4 mM. In the presence of external bicarbonate, amiloride or omission of sodium slowed, but did not completely inhibit recovery from acidification, indicating that additional pHi regulation mechanisms may operate in this preparation. These studies provide a direct measurement of pHi in hepatocyte couplets and indicate that Na-H exchange, together with a bicarbonate dependent system are important mechanisms for pHi regulation in this preparation.
R M Henderson, and
J Graf, and
J L Boyer
August 1995,
The Journal of clinical investigation,
R M Henderson, and
J Graf, and
J L Boyer
July 1992,
Pflugers Archiv : European journal of physiology,
R M Henderson, and
J Graf, and
J L Boyer
January 1991,
The American journal of physiology,
R M Henderson, and
J Graf, and
J L Boyer
January 1993,
Biochemical pharmacology,
R M Henderson, and
J Graf, and
J L Boyer
January 1989,
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R M Henderson, and
J Graf, and
J L Boyer
October 1989,
Gastroenterology,
R M Henderson, and
J Graf, and
J L Boyer
January 1985,
Transactions of the Association of American Physicians,
R M Henderson, and
J Graf, and
J L Boyer
November 1988,
Journal of cellular physiology,
R M Henderson, and
J Graf, and
J L Boyer
May 1989,
Wiener klinische Wochenschrift,
R M Henderson, and
J Graf, and
J L Boyer
November 1995,
Gastroenterology,
