-transparent.png){kind=logo}
Structural Studies of Amyloid Fibrils by Paramagnetic Solid-State Nuclear Magnetic Resonance Spectroscopy. 2018
Theint Theint, and
Yongjie Xia, and
Philippe S Nadaud, and
Dwaipayan Mukhopadhyay, and
Charles D Schwieters, and
Krystyna Surewicz, and
Witold K Surewicz, and
Christopher P Jaroniec
Department of Chemistry and Biochemistry , The Ohio State University , Columbus , Ohio 43210 , United States.
Application of paramagnetic solid-state NMR to amyloids is demonstrated, using Y145Stop human prion protein modified with nitroxide spin-label or EDTA-Cu2+ tags as a model. By using sample preparation protocols based on seeding with preformed fibrils, we show that paramagnetic protein analogs can be induced into adopting the wild-type amyloid structure. Measurements of residue-specific intramolecular and intermolecular paramagnetic relaxation enhancements enable determination of protein fold within the fibril core and protofilament assembly. These methods are expected to be widely applicable to other amyloids and protein assemblies.
Related Publications
Theint Theint, and
Yongjie Xia, and
Philippe S Nadaud, and
Dwaipayan Mukhopadhyay, and
Charles D Schwieters, and
Krystyna Surewicz, and
Witold K Surewicz, and
Christopher P Jaroniec
January 2001,
Methods in enzymology,
Theint Theint, and
Yongjie Xia, and
Philippe S Nadaud, and
Dwaipayan Mukhopadhyay, and
Charles D Schwieters, and
Krystyna Surewicz, and
Witold K Surewicz, and
Christopher P Jaroniec
January 2012,
Solid state nuclear magnetic resonance,
Theint Theint, and
Yongjie Xia, and
Philippe S Nadaud, and
Dwaipayan Mukhopadhyay, and
Charles D Schwieters, and
Krystyna Surewicz, and
Witold K Surewicz, and
Christopher P Jaroniec
February 2010,
Journal of the American Chemical Society,
Theint Theint, and
Yongjie Xia, and
Philippe S Nadaud, and
Dwaipayan Mukhopadhyay, and
Charles D Schwieters, and
Krystyna Surewicz, and
Witold K Surewicz, and
Christopher P Jaroniec
September 2017,
Journal of visualized experiments : JoVE,
Theint Theint, and
Yongjie Xia, and
Philippe S Nadaud, and
Dwaipayan Mukhopadhyay, and
Charles D Schwieters, and
Krystyna Surewicz, and
Witold K Surewicz, and
Christopher P Jaroniec
August 2007,
The journal of physical chemistry. B,
Theint Theint, and
Yongjie Xia, and
Philippe S Nadaud, and
Dwaipayan Mukhopadhyay, and
Charles D Schwieters, and
Krystyna Surewicz, and
Witold K Surewicz, and
Christopher P Jaroniec
January 1989,
Methods in enzymology,
Theint Theint, and
Yongjie Xia, and
Philippe S Nadaud, and
Dwaipayan Mukhopadhyay, and
Charles D Schwieters, and
Krystyna Surewicz, and
Witold K Surewicz, and
Christopher P Jaroniec
December 2002,
Biochemistry,
Theint Theint, and
Yongjie Xia, and
Philippe S Nadaud, and
Dwaipayan Mukhopadhyay, and
Charles D Schwieters, and
Krystyna Surewicz, and
Witold K Surewicz, and
Christopher P Jaroniec
April 2015,
Journal of magnetic resonance (San Diego, Calif. : 1997),
Theint Theint, and
Yongjie Xia, and
Philippe S Nadaud, and
Dwaipayan Mukhopadhyay, and
Charles D Schwieters, and
Krystyna Surewicz, and
Witold K Surewicz, and
Christopher P Jaroniec
June 2020,
Biochemistry,
Theint Theint, and
Yongjie Xia, and
Philippe S Nadaud, and
Dwaipayan Mukhopadhyay, and
Charles D Schwieters, and
Krystyna Surewicz, and
Witold K Surewicz, and
Christopher P Jaroniec
March 2012,
Proceedings of the National Academy of Sciences of the United States of America,
