BIOMAT Database Logo BIOMAT Database Logo

Production of succinate by recombinant Escherichia coli using acetate as the sole carbon source. 2018

Hao Niu, and Ruirui Li, and Jing Wu, and Zhaohui Cai, and Danxiong Yang, and Pengfei Gu, and Qiang Li
School of Biological Science and Technology, University of Jinan, Jinan, 250022 People's Republic of China.

Acetate is a potential low-cost carbon source that can be generated by biological and chemical processes. In this study, deletion of sdhAB encoding succinate dehydrogenase, iclR encoding the isocitrate lyase regulator, and maeB encoding the malic enzyme, and overexpression of acs encoding acetyl-CoA synthetase, gltA encoding citrate synthase, and acnB encoding aconitate hydratase in the wild-type Escherichia coli MG1655 strain yielded the recombinant E. coli strain WCY-7, which could synthesize succinate from acetate. After 48 h batch fermentation, this strain accumulated 11.23 mM succinate from 50 mM sodium acetate. This work indicates that microbial fermentation using acetate as the sole carbon source may be a suitable route to produce high yields of the valuable chemicals.

UI MeSH Term Description Entries

Related Publications

Hao Niu, and Ruirui Li, and Jing Wu, and Zhaohui Cai, and Danxiong Yang, and Pengfei Gu, and Qiang Li
The utilization of acetate-C14 by Escherichia coli grown on acetate as the sole carbon source.
August 1959, The Journal of biological chemistry,
Hao Niu, and Ruirui Li, and Jing Wu, and Zhaohui Cai, and Danxiong Yang, and Pengfei Gu, and Qiang Li
Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source.
May 2013, Bioresource technology,
Hao Niu, and Ruirui Li, and Jing Wu, and Zhaohui Cai, and Danxiong Yang, and Pengfei Gu, and Qiang Li
Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source.
January 2018, Bioengineered,
Hao Niu, and Ruirui Li, and Jing Wu, and Zhaohui Cai, and Danxiong Yang, and Pengfei Gu, and Qiang Li
Process development of succinic acid production by Escherichia coli NZN111 using acetate as an aerobic carbon source.
October 2011, Enzyme and microbial technology,
Hao Niu, and Ruirui Li, and Jing Wu, and Zhaohui Cai, and Danxiong Yang, and Pengfei Gu, and Qiang Li
Engineering Escherichia coli to Utilize Erythritol as Sole Carbon Source.
May 2023, Advanced science (Weinheim, Baden-Wurttemberg, Germany),
Hao Niu, and Ruirui Li, and Jing Wu, and Zhaohui Cai, and Danxiong Yang, and Pengfei Gu, and Qiang Li
Production of Succinate from Acetate by Metabolically Engineered Escherichia coli.
November 2016, ACS synthetic biology,
Hao Niu, and Ruirui Li, and Jing Wu, and Zhaohui Cai, and Danxiong Yang, and Pengfei Gu, and Qiang Li
Production of targeted poly(3-hydroxyalkanoates) copolymers by glycogen accumulating organisms using acetate as sole carbon source.
May 2007, Journal of biotechnology,
Hao Niu, and Ruirui Li, and Jing Wu, and Zhaohui Cai, and Danxiong Yang, and Pengfei Gu, and Qiang Li
Effective production of low crystallinity Poly(3-hydroxybutyrate) by recombinant E. coli strain JM109 using crude glycerol as sole carbon source.
September 2015, Bioresource technology,
Hao Niu, and Ruirui Li, and Jing Wu, and Zhaohui Cai, and Danxiong Yang, and Pengfei Gu, and Qiang Li
Acetate: friend or foe? Efficient production of a sweet protein in Escherichia coli BL21 using acetate as a carbon source.
July 2015, Microbial cell factories,
Hao Niu, and Ruirui Li, and Jing Wu, and Zhaohui Cai, and Danxiong Yang, and Pengfei Gu, and Qiang Li
[Elimination of succinate and acetate synthesis in recombinant Escherichia coli for D-lactate production].
January 2011, Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
Copied contents to your clipboard!