BIOMAT Database Logo BIOMAT Database Logo

[Effect of promoter duplication in the synthetic interferon gene on the level of its expression]. 1987

A A Il'ichev, and N V Tikunova, and O I Serpinskiĭ, and I V Timofeev, and S N Shchelkunov

The expression of the artificial gene for the human leukocyte interferon (IFN) synthesized by the chemo-enzymic method has been studied in Escherichia coli cells. The genetic constructions in which the IFN gene transcription is carried out from A2 and B promoters of T7 phage or from E. coli lacZ UV5 gene promoter have been obtained, based on pEMB101 plasmid. In addition, the level of interferon production, depending on the promoter used, has been studied. It has been shown that the tandemly located promoters increase efficiency of the IFN gene expression.

UI MeSH Term Description Entries
D007370 Interferon Type I Interferon secreted by leukocytes, fibroblasts, or lymphoblasts in response to viruses or interferon inducers other than mitogens, antigens, or allo-antigens. They include alpha- and beta-interferons (INTERFERON-ALPHA and INTERFERON-BETA). Interferons Type I,Type I Interferon,Type I Interferons,Interferon, Type I,Interferons, Type I
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011401 Promoter Regions, Genetic DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. rRNA Promoter,Early Promoters, Genetic,Late Promoters, Genetic,Middle Promoters, Genetic,Promoter Regions,Promoter, Genetic,Promotor Regions,Promotor, Genetic,Pseudopromoter, Genetic,Early Promoter, Genetic,Genetic Late Promoter,Genetic Middle Promoters,Genetic Promoter,Genetic Promoter Region,Genetic Promoter Regions,Genetic Promoters,Genetic Promotor,Genetic Promotors,Genetic Pseudopromoter,Genetic Pseudopromoters,Late Promoter, Genetic,Middle Promoter, Genetic,Promoter Region,Promoter Region, Genetic,Promoter, Genetic Early,Promoter, rRNA,Promoters, Genetic,Promoters, Genetic Middle,Promoters, rRNA,Promotor Region,Promotors, Genetic,Pseudopromoters, Genetic,Region, Genetic Promoter,Region, Promoter,Region, Promotor,Regions, Genetic Promoter,Regions, Promoter,Regions, Promotor,rRNA Promoters
D004262 DNA Restriction Enzymes Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D005810 Multigene Family A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed) Gene Clusters,Genes, Reiterated,Cluster, Gene,Clusters, Gene,Families, Multigene,Family, Multigene,Gene Cluster,Gene, Reiterated,Multigene Families,Reiterated Gene,Reiterated Genes
D005813 Genes, Synthetic Biologically functional sequences of DNA chemically synthesized in vitro. Artificial Genes,Synthetic Genes,Artificial Gene,Gene, Artificial,Gene, Synthetic,Genes, Artificial,Synthetic Gene
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013604 T-Phages A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA. Bacteriophages T,Coliphages T,Phages T,T Phages,T-Phage

Related Publications

A A Il'ichev, and N V Tikunova, and O I Serpinskiĭ, and I V Timofeev, and S N Shchelkunov
The construction of a synthetic Escherichia coli trp promoter and its use in the expression of a synthetic interferon gene.
November 1982, Nucleic acids research,
A A Il'ichev, and N V Tikunova, and O I Serpinskiĭ, and I V Timofeev, and S N Shchelkunov
Expression of human interferon-α8 synthetic gene under P(BAD) promoter.
October 2012, Biochemistry. Biokhimiia,
A A Il'ichev, and N V Tikunova, and O I Serpinskiĭ, and I V Timofeev, and S N Shchelkunov
[Duplication of a synthetic gene for human leukocyte interferon and its expression in polycistron mRNA with coupled translation system].
September 1987, Bioorganicheskaia khimiia,
A A Il'ichev, and N V Tikunova, and O I Serpinskiĭ, and I V Timofeev, and S N Shchelkunov
Synthetic promoter libraries--tuning of gene expression.
February 2006, Trends in biotechnology,
A A Il'ichev, and N V Tikunova, and O I Serpinskiĭ, and I V Timofeev, and S N Shchelkunov
Construction of multipurpose gene cartridges based on a novel synthetic promoter for high-level gene expression in gram-negative bacteria.
June 1994, Gene,
A A Il'ichev, and N V Tikunova, and O I Serpinskiĭ, and I V Timofeev, and S N Shchelkunov
LATS2 promoter hypermethylation and its effect on gene expression in human breast cancer.
February 2018, Oncology letters,
A A Il'ichev, and N V Tikunova, and O I Serpinskiĭ, and I V Timofeev, and S N Shchelkunov
Regulatory Effect of Methylation of the Porcine AQP3 Gene Promoter Region on Its Expression Level and Porcine Epidemic Diarrhea Virus Resistance.
October 2020, Genes,
A A Il'ichev, and N V Tikunova, and O I Serpinskiĭ, and I V Timofeev, and S N Shchelkunov
Mouse Mx2 gene: organization, mRNA expression and the role of the interferon-response promoter in its regulation.
March 2003, Gene,
A A Il'ichev, and N V Tikunova, and O I Serpinskiĭ, and I V Timofeev, and S N Shchelkunov
Characterizing microRNA-mediated modulation of gene expression noise and its effect on synthetic gene circuits.
August 2021, Cell reports,
A A Il'ichev, and N V Tikunova, and O I Serpinskiĭ, and I V Timofeev, and S N Shchelkunov
T-786C variation in the promoter sequence of human eNOS gene markedly influences its expression level.
January 2017, Drug discoveries & therapeutics,
Copied contents to your clipboard!