Spin label EPR suggests the presence of cholesterol rich domains in cultured insect cell membranes. 2018

M A Baqué, and V V Gioria, and G A Micheloud, and N M C Casado, and J D Claus, and A M Gennaro
Departamento de Física, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional de Litoral, Ciudad Universitaria, 3000, Santa Fe, Argentina.

Different spin labels were incorporated to the membranes of cultured insect UFL-AG-286 cells in order to characterize their physical properties by Electron Paramagnetic Resonance spectroscopy (EPR). The spectrum of the spin label 12-SASL incorporated to cell membranes was similar as those obtained in membrane model systems composed of eggPC/cholesterol. However, the spectrum of the spin label CSL, chemically related to cholesterol, was drastically different in the two systems. Interestingly, when cell cholesterol content was reduced using methyl beta cyclodextrin, an EPR spectrum similar to those of model membranes was obtained. The analysis of these experiments suggests the existence of cholesterol rich regions in UFL-AG-286 cell membranes.

UI MeSH Term Description Entries
D007915 Lepidoptera A large order of insects comprising the butterflies and moths.
D008055 Lipids A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed) Lipid
D008081 Liposomes Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins. Niosomes,Transferosomes,Ultradeformable Liposomes,Liposomes, Ultra-deformable,Liposome,Liposome, Ultra-deformable,Liposome, Ultradeformable,Liposomes, Ultra deformable,Liposomes, Ultradeformable,Niosome,Transferosome,Ultra-deformable Liposome,Ultra-deformable Liposomes,Ultradeformable Liposome
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002784 Cholesterol The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils. Epicholesterol
D004578 Electron Spin Resonance Spectroscopy A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING. ENDOR,Electron Nuclear Double Resonance,Electron Paramagnetic Resonance,Paramagnetic Resonance,Electron Spin Resonance,Paramagnetic Resonance, Electron,Resonance, Electron Paramagnetic,Resonance, Electron Spin,Resonance, Paramagnetic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013113 Spin Labels Molecules which contain an atom or a group of atoms exhibiting an unpaired electron spin that can be detected by electron spin resonance spectroscopy and can be bonded to another molecule. (McGraw-Hill Dictionary of Chemical and Technical Terms, 4th ed) Spin Label,Label, Spin,Labels, Spin

Related Publications

M A Baqué, and V V Gioria, and G A Micheloud, and N M C Casado, and J D Claus, and A M Gennaro
January 2003, Chemistry and physics of lipids,
M A Baqué, and V V Gioria, and G A Micheloud, and N M C Casado, and J D Claus, and A M Gennaro
January 2004, Biophysical journal,
M A Baqué, and V V Gioria, and G A Micheloud, and N M C Casado, and J D Claus, and A M Gennaro
November 2005, Magnetic resonance in chemistry : MRC,
M A Baqué, and V V Gioria, and G A Micheloud, and N M C Casado, and J D Claus, and A M Gennaro
October 1998, Proceedings of the National Academy of Sciences of the United States of America,
M A Baqué, and V V Gioria, and G A Micheloud, and N M C Casado, and J D Claus, and A M Gennaro
February 2012, European biophysics journal : EBJ,
M A Baqué, and V V Gioria, and G A Micheloud, and N M C Casado, and J D Claus, and A M Gennaro
April 2006, Chemistry and physics of lipids,
M A Baqué, and V V Gioria, and G A Micheloud, and N M C Casado, and J D Claus, and A M Gennaro
June 2020, Cell biochemistry and biophysics,
M A Baqué, and V V Gioria, and G A Micheloud, and N M C Casado, and J D Claus, and A M Gennaro
January 2005, Journal of molecular biology,
M A Baqué, and V V Gioria, and G A Micheloud, and N M C Casado, and J D Claus, and A M Gennaro
May 1982, American journal of human genetics,
M A Baqué, and V V Gioria, and G A Micheloud, and N M C Casado, and J D Claus, and A M Gennaro
August 2013, The journal of physical chemistry. B,
Copied contents to your clipboard!