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Differentiated primary rat hepatocyte cultures have been infected with retroviral vectors expressing human hypoxanthine/guanine phosphoribosyltransferase or the transposon Tn5 neomycin-resistance gene. Expression of the markers was detected only after infection of the cells during a short period of cell replication and transient dedifferentiation from days 1 to 5 of culture. Provirus integrated during that period remains fully expressed during the entire subsequent stationary period of culture up to at least 25 days. Selection with the neomycin analogue G418 of cells infected with the neomycin vector led to the appearance of cells with hepatocyte morphology in which newly synthesized albumin was detectable by immunoprecipitation, indicating successful infection of hepatocytes.
J A Wolff, and
J K Yee, and
H F Skelly, and
J C Moores, and
J G Respess, and
T Friedmann, and
H Leffert
April 1983,
In vitro,
J A Wolff, and
J K Yee, and
H F Skelly, and
J C Moores, and
J G Respess, and
T Friedmann, and
H Leffert
January 1980,
Annals of the New York Academy of Sciences,
J A Wolff, and
J K Yee, and
H F Skelly, and
J C Moores, and
J G Respess, and
T Friedmann, and
H Leffert
April 2006,
The journal of gene medicine,
J A Wolff, and
J K Yee, and
H F Skelly, and
J C Moores, and
J G Respess, and
T Friedmann, and
H Leffert
November 1996,
Biochemical pharmacology,
J A Wolff, and
J K Yee, and
H F Skelly, and
J C Moores, and
J G Respess, and
T Friedmann, and
H Leffert
June 1986,
The Journal of biological chemistry,
J A Wolff, and
J K Yee, and
H F Skelly, and
J C Moores, and
J G Respess, and
T Friedmann, and
H Leffert
May 1986,
Biochemical pharmacology,
J A Wolff, and
J K Yee, and
H F Skelly, and
J C Moores, and
J G Respess, and
T Friedmann, and
H Leffert
January 1989,
Biochemical pharmacology,
J A Wolff, and
J K Yee, and
H F Skelly, and
J C Moores, and
J G Respess, and
T Friedmann, and
H Leffert
December 1979,
The Journal of biological chemistry,
J A Wolff, and
J K Yee, and
H F Skelly, and
J C Moores, and
J G Respess, and
T Friedmann, and
H Leffert
January 1996,
Bollettino della Societa italiana di biologia sperimentale,
J A Wolff, and
J K Yee, and
H F Skelly, and
J C Moores, and
J G Respess, and
T Friedmann, and
H Leffert
September 1975,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
