-transparent.png){kind=logo}
Low-affinity receptors (Fc epsilon R) and secreted factors (IgE-BF) which bind to immunoglobulins of the IgE isotype play a key role in the regulation of human IgE synthesis. We report here the cloning of a cDNA coding for the Fc epsilon R of the human B-lymphoblast cell line RPMI 8866. The nucleotide sequence of this cDNA predicts a polypeptide with 321 amino acids and a mol. wt of 36,281 daltons. A functional Fc epsilon R capable of binding IgE was expressed in Chinese hamster ovary cells after stable transformation with the cDNA which had been cloned into a mammalian expression vector. Amino acid sequence analysis of IgE-BF purified from RPMI 8866 cells revealed an amino-terminal sequence of 19 residues which coincides with the predicted amino acid sequence of the Fc epsilon R, starting at residues 148 and 150. A computer search with the translated amino acid sequence of the Fc epsilon R revealed a domain of 120 amino acids having striking homology to the human asialoglycoprotein receptors.
C Lüdin, and
H Hofstetter, and
M Sarfati, and
C A Levy, and
U Suter, and
D Alaimo, and
E Kilchherr, and
H Frost, and
G Delespesse
May 1994,
FEBS letters,
C Lüdin, and
H Hofstetter, and
M Sarfati, and
C A Levy, and
U Suter, and
D Alaimo, and
E Kilchherr, and
H Frost, and
G Delespesse
March 2002,
European journal of biochemistry,
C Lüdin, and
H Hofstetter, and
M Sarfati, and
C A Levy, and
U Suter, and
D Alaimo, and
E Kilchherr, and
H Frost, and
G Delespesse
January 1989,
International immunology,
C Lüdin, and
H Hofstetter, and
M Sarfati, and
C A Levy, and
U Suter, and
D Alaimo, and
E Kilchherr, and
H Frost, and
G Delespesse
November 1993,
Molecular pharmacology,
C Lüdin, and
H Hofstetter, and
M Sarfati, and
C A Levy, and
U Suter, and
D Alaimo, and
E Kilchherr, and
H Frost, and
G Delespesse
April 1994,
The Journal of biological chemistry,
C Lüdin, and
H Hofstetter, and
M Sarfati, and
C A Levy, and
U Suter, and
D Alaimo, and
E Kilchherr, and
H Frost, and
G Delespesse
January 1994,
The Journal of biological chemistry,
C Lüdin, and
H Hofstetter, and
M Sarfati, and
C A Levy, and
U Suter, and
D Alaimo, and
E Kilchherr, and
H Frost, and
G Delespesse
February 1991,
Nature,
C Lüdin, and
H Hofstetter, and
M Sarfati, and
C A Levy, and
U Suter, and
D Alaimo, and
E Kilchherr, and
H Frost, and
G Delespesse
May 1988,
Proceedings of the National Academy of Sciences of the United States of America,
C Lüdin, and
H Hofstetter, and
M Sarfati, and
C A Levy, and
U Suter, and
D Alaimo, and
E Kilchherr, and
H Frost, and
G Delespesse
June 1995,
FEBS letters,
C Lüdin, and
H Hofstetter, and
M Sarfati, and
C A Levy, and
U Suter, and
D Alaimo, and
E Kilchherr, and
H Frost, and
G Delespesse
February 1986,
Science (New York, N.Y.),
