-transparent.png){kind=logo}
Muscarinic receptors have been purified from calf forebrain plasma cell membranes by affinity chromatography on a dexetimide-agarose gel. SDS-PAGE analysis showed a single 70 kDa band. Monoclonal antibodies have been prepared against these affinity purified 70 kDa protein(s). One antibody, M-35, immunoprecipitated up to 80% of digitonin-solubilized muscarinic receptors. M-35 had agonist-like effects on guinea-pig myometrium: it increased the intracellular cyclic GMP content, decreased prostaglandin-induced cyclic AMP accumulation and caused muscle contractions. The two first effects were inhibited by atropine. M-35 was used to visualize muscarinic receptors at the surface of human fibroblastic cells. In the particular cell line used, the receptors have a low affinity for pirenzepine, were negatively coupled to adenylate cyclase and mediated increase in the phosphatidyl-inositol breakdown.
C André, and
S Marullo, and
J G Guillet, and
A Convents, and
M Lauwereys, and
S Kaveri, and
J Hoebeke, and
A D Strosberg
January 1988,
Advances in experimental medicine and biology,
C André, and
S Marullo, and
J G Guillet, and
A Convents, and
M Lauwereys, and
S Kaveri, and
J Hoebeke, and
A D Strosberg
January 2003,
Receptors & channels,
C André, and
S Marullo, and
J G Guillet, and
A Convents, and
M Lauwereys, and
S Kaveri, and
J Hoebeke, and
A D Strosberg
February 1977,
Immunochemistry,
C André, and
S Marullo, and
J G Guillet, and
A Convents, and
M Lauwereys, and
S Kaveri, and
J Hoebeke, and
A D Strosberg
March 1990,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
C André, and
S Marullo, and
J G Guillet, and
A Convents, and
M Lauwereys, and
S Kaveri, and
J Hoebeke, and
A D Strosberg
January 1978,
Progress in neurobiology,
C André, and
S Marullo, and
J G Guillet, and
A Convents, and
M Lauwereys, and
S Kaveri, and
J Hoebeke, and
A D Strosberg
February 1977,
Archives of general psychiatry,
C André, and
S Marullo, and
J G Guillet, and
A Convents, and
M Lauwereys, and
S Kaveri, and
J Hoebeke, and
A D Strosberg
July 1991,
Biochemical pharmacology,
C André, and
S Marullo, and
J G Guillet, and
A Convents, and
M Lauwereys, and
S Kaveri, and
J Hoebeke, and
A D Strosberg
January 1987,
Annual review of neuroscience,
C André, and
S Marullo, and
J G Guillet, and
A Convents, and
M Lauwereys, and
S Kaveri, and
J Hoebeke, and
A D Strosberg
November 1983,
The Journal of biological chemistry,
C André, and
S Marullo, and
J G Guillet, and
A Convents, and
M Lauwereys, and
S Kaveri, and
J Hoebeke, and
A D Strosberg
December 1993,
Annals of the New York Academy of Sciences,
