-transparent.png){kind=logo}
The role of prostaglandin I2 (PGI2) in the control of DNA synthesis during the cell cycle was investigated in cultured rabbit aortic smooth muscle cells (SMC). SMC at confluency in the G0 state reached the S phase about 16 h after stimulation with serum, as judged by measurement of [3H]thymidine incorporation into DNA (DNA synthesis). Cyclooxygenase inhibitors such as indomethacin and aspirin enhanced DNA synthesis, suggesting that endogenously synthesized prostaglandins inhibit DNA synthesis. Added PGE1 or PGE2 had little effect on DNA synthesis. PGI2 inhibited DNA synthesis only when added from 10 to 16 h after stimulation of SMC in the G0 state with serum. Addition of CS-570, a stable PGI2 analogue, inhibited DNA synthesis at any time after serum stimulation. The endogenous syntheses of PGI2 and DNA were negatively correlated. These results suggest that PGI2 inhibits DNA synthesis by acting on the progression stage of the G1 state.
N Morisaki, and
T Kanzaki, and
N Motoyama, and
Y Saito, and
S Yoshida
June 1987,
FEBS letters,
N Morisaki, and
T Kanzaki, and
N Motoyama, and
Y Saito, and
S Yoshida
January 1990,
The Biochemical journal,
N Morisaki, and
T Kanzaki, and
N Motoyama, and
Y Saito, and
S Yoshida
September 1985,
Experimental cell research,
N Morisaki, and
T Kanzaki, and
N Motoyama, and
Y Saito, and
S Yoshida
April 1980,
Biochemistry,
N Morisaki, and
T Kanzaki, and
N Motoyama, and
Y Saito, and
S Yoshida
March 1983,
Gastroenterology,
N Morisaki, and
T Kanzaki, and
N Motoyama, and
Y Saito, and
S Yoshida
February 1988,
Experimental and molecular pathology,
N Morisaki, and
T Kanzaki, and
N Motoyama, and
Y Saito, and
S Yoshida
March 1989,
The American journal of physiology,
N Morisaki, and
T Kanzaki, and
N Motoyama, and
Y Saito, and
S Yoshida
July 1988,
The Journal of cell biology,
N Morisaki, and
T Kanzaki, and
N Motoyama, and
Y Saito, and
S Yoshida
January 1993,
Pathobiology : journal of immunopathology, molecular and cellular biology,
N Morisaki, and
T Kanzaki, and
N Motoyama, and
Y Saito, and
S Yoshida
March 1982,
Biochimica et biophysica acta,
