Deoxynivalenol Affects Cell Metabolism and Increases Protein Biosynthesis in Intestinal Porcine Epithelial Cells (IPEC-J2): DON Increases Protein Biosynthesis. 2018

Constanze Nossol, and Peter Landgraf, and Stefan Kahlert, and Michael Oster, and Berend Isermann, and Daniela C Dieterich, and Klaus Wimmers, and Sven Dänicke, and Hermann-Josef Rothkötter
Medical Faculty, Institute of Anatomy, Otto-von-Guericke University, 39120 Magdeburg, Germany. constanze.nossol@med.ovgu.de.

Deoxynivalenol (DON) is a toxin found in cereals as well as in processed products such as pasta, and causes substantial economic losses for stock breeding as it induces vomiting, reduced feeding, and reduced growth rates in piglets. Oxidative phosphorylation, TCA-cycle, transcription, and translation have been hypothesized to be leading pathways that are affected by DON. We used an application of high and low glucose to examine oxidative phosphorylation and anaerobic glycolysis. A change in the metabolic status of IPEC-J2 was observed and confirmed by microarray data. Measurements of oxygen consumption resulted in a significant reduction, if DON attacks from the basolateral. Furthermore, we found a dose-dependent effect with a significant reduction at 2000 ng/mL. In addition, SLC7A11 and PHB, the genes with the highest regulation in our microarray analyses under low glucose supply, were investigated and showed a variable regulation on protein level. Lactate production and glucose consumption was investigated to examine the impact of DON on anaerobic glycolysis and we observed a significant increase in 2000 blhigh and a decrease in 2000 aphigh. Interestingly, both groups as well as 200 blhigh showed a significant higher de novo protein synthesis when compared to the control. These results indicate the direct or indirect impact of DON on metabolic pathways in IPEC-J2.

UI MeSH Term Description Entries
D007422 Intestines The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE. Intestine
D010085 Oxidative Phosphorylation Electron transfer through the cytochrome system liberating free energy which is transformed into high-energy phosphate bonds. Phosphorylation, Oxidative,Oxidative Phosphorylations,Phosphorylations, Oxidative
D010101 Oxygen Consumption The rate at which oxygen is used by a tissue; microliters of oxygen STPD used per milligram of tissue per hour; the rate at which oxygen enters the blood from alveolar gas, equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body. (Stedman, 25th ed, p346) Consumption, Oxygen,Consumptions, Oxygen,Oxygen Consumptions
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004847 Epithelial Cells Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells. Adenomatous Epithelial Cells,Columnar Glandular Epithelial Cells,Cuboidal Glandular Epithelial Cells,Glandular Epithelial Cells,Squamous Cells,Squamous Epithelial Cells,Transitional Epithelial Cells,Adenomatous Epithelial Cell,Cell, Adenomatous Epithelial,Cell, Epithelial,Cell, Glandular Epithelial,Cell, Squamous,Cell, Squamous Epithelial,Cell, Transitional Epithelial,Cells, Adenomatous Epithelial,Cells, Epithelial,Cells, Glandular Epithelial,Cells, Squamous,Cells, Squamous Epithelial,Cells, Transitional Epithelial,Epithelial Cell,Epithelial Cell, Adenomatous,Epithelial Cell, Glandular,Epithelial Cell, Squamous,Epithelial Cell, Transitional,Epithelial Cells, Adenomatous,Epithelial Cells, Glandular,Epithelial Cells, Squamous,Epithelial Cells, Transitional,Glandular Epithelial Cell,Squamous Cell,Squamous Epithelial Cell,Transitional Epithelial Cell
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D005947 Glucose A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement. Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D006019 Glycolysis A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH. Embden-Meyerhof Pathway,Embden-Meyerhof-Parnas Pathway,Embden Meyerhof Parnas Pathway,Embden Meyerhof Pathway,Embden-Meyerhof Pathways,Pathway, Embden-Meyerhof,Pathway, Embden-Meyerhof-Parnas,Pathways, Embden-Meyerhof
D000255 Adenosine Triphosphate An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter. ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Constanze Nossol, and Peter Landgraf, and Stefan Kahlert, and Michael Oster, and Berend Isermann, and Daniela C Dieterich, and Klaus Wimmers, and Sven Dänicke, and Hermann-Josef Rothkötter
January 2011, Toxicology letters,
Constanze Nossol, and Peter Landgraf, and Stefan Kahlert, and Michael Oster, and Berend Isermann, and Daniela C Dieterich, and Klaus Wimmers, and Sven Dänicke, and Hermann-Josef Rothkötter
October 2020, Journal of applied toxicology : JAT,
Constanze Nossol, and Peter Landgraf, and Stefan Kahlert, and Michael Oster, and Berend Isermann, and Daniela C Dieterich, and Klaus Wimmers, and Sven Dänicke, and Hermann-Josef Rothkötter
May 2012, Veterinary microbiology,
Constanze Nossol, and Peter Landgraf, and Stefan Kahlert, and Michael Oster, and Berend Isermann, and Daniela C Dieterich, and Klaus Wimmers, and Sven Dänicke, and Hermann-Josef Rothkötter
January 2010, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
Constanze Nossol, and Peter Landgraf, and Stefan Kahlert, and Michael Oster, and Berend Isermann, and Daniela C Dieterich, and Klaus Wimmers, and Sven Dänicke, and Hermann-Josef Rothkötter
November 2019, Toxins,
Constanze Nossol, and Peter Landgraf, and Stefan Kahlert, and Michael Oster, and Berend Isermann, and Daniela C Dieterich, and Klaus Wimmers, and Sven Dänicke, and Hermann-Josef Rothkötter
November 2014, Virology journal,
Constanze Nossol, and Peter Landgraf, and Stefan Kahlert, and Michael Oster, and Berend Isermann, and Daniela C Dieterich, and Klaus Wimmers, and Sven Dänicke, and Hermann-Josef Rothkötter
December 2018, Toxins,
Constanze Nossol, and Peter Landgraf, and Stefan Kahlert, and Michael Oster, and Berend Isermann, and Daniela C Dieterich, and Klaus Wimmers, and Sven Dänicke, and Hermann-Josef Rothkötter
August 2012, Journal of animal physiology and animal nutrition,
Constanze Nossol, and Peter Landgraf, and Stefan Kahlert, and Michael Oster, and Berend Isermann, and Daniela C Dieterich, and Klaus Wimmers, and Sven Dänicke, and Hermann-Josef Rothkötter
January 2012, PloS one,
Constanze Nossol, and Peter Landgraf, and Stefan Kahlert, and Michael Oster, and Berend Isermann, and Daniela C Dieterich, and Klaus Wimmers, and Sven Dänicke, and Hermann-Josef Rothkötter
April 2017, In vitro cellular & developmental biology. Animal,
Copied contents to your clipboard!