A high resolution diffracting crystal form of the complex between yeast tRNAAsp and aspartyl-tRNA synthetase. 1988

M Ruff, and J Cavarelli, and V Mikol, and B Lorber, and A Mitschler, and R Giege, and J C Thierry, and D Moras
Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.

Three new crystal forms of the complex between yeast tRNAAsp and aspartyl-tRNA synthetase have been produced. The best crystals, obtained after modifying both purification and crystallization conditions, belong to space group P2(1)2(1)2(1) and diffract to 2.7 A. Unit cell parameters are a = 210.4 A, b = 145.3 A and c = 86.0 A (1 A = 0.1 nm), with one dimeric enzyme and two tRNA molecules in the asymmetric unit.

UI MeSH Term Description Entries
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D000604 Amino Acyl-tRNA Synthetases A subclass of enzymes that aminoacylate AMINO ACID-SPECIFIC TRANSFER RNA with their corresponding AMINO ACIDS. Amino Acyl T RNA Synthetases,Amino Acyl-tRNA Ligases,Aminoacyl Transfer RNA Synthetase,Aminoacyl-tRNA Synthetase,Transfer RNA Synthetase,tRNA Synthetase,Acyl-tRNA Ligases, Amino,Acyl-tRNA Synthetases, Amino,Amino Acyl tRNA Ligases,Amino Acyl tRNA Synthetases,Aminoacyl tRNA Synthetase,Ligases, Amino Acyl-tRNA,RNA Synthetase, Transfer,Synthetase, Aminoacyl-tRNA,Synthetase, Transfer RNA,Synthetase, tRNA,Synthetases, Amino Acyl-tRNA
D001226 Aspartate-tRNA Ligase An enzyme that activates aspartic acid with its specific transfer RNA. EC 6.1.1.12. Aspartyl T RNA Synthetase,Asp-tRNA Ligase,Aspartyl-tRNA Synthetase,Asp tRNA Ligase,Aspartate tRNA Ligase,Aspartyl tRNA Synthetase,Ligase, Asp-tRNA,Ligase, Aspartate-tRNA,Synthetase, Aspartyl-tRNA
D012331 RNA, Fungal Ribonucleic acid in fungi having regulatory and catalytic roles as well as involvement in protein synthesis. Fungal RNA
D012345 RNA, Transfer, Amino Acid-Specific A group of transfer RNAs which are specific for carrying each one of the 20 amino acids to the ribosome in preparation for protein synthesis. Amino Acid-Specific Transfer RNA,Amino Acid-Specific tRNA,Transfer RNA, Amino Acid-Specific,tRNA-Amino Acid,RNA, Transfer, Amino Acid Specific,tRNA (Amino Acid),Acid, tRNA-Amino,Acid-Specific tRNA, Amino,Amino Acid Specific Transfer RNA,Amino Acid Specific tRNA,Transfer RNA, Amino Acid Specific,tRNA Amino Acid,tRNA, Amino Acid-Specific
D012349 RNA, Transfer, Asp A transfer RNA which is specific for carrying aspartic acid to sites on the ribosomes in preparation for protein synthesis. Aspartic Acid-Specific tRNA,Transfer RNA, Asp,tRNAAsp,tRNA(Asp),Acid-Specific tRNA, Aspartic,Asp Transfer RNA,Aspartic Acid Specific tRNA,RNA, Asp Transfer,tRNA, Aspartic Acid-Specific
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D014961 X-Ray Diffraction The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Xray Diffraction,Diffraction, X-Ray,Diffraction, Xray,Diffractions, X-Ray,Diffractions, Xray,X Ray Diffraction,X-Ray Diffractions,Xray Diffractions

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