BIOMAT Database Logo BIOMAT Database Logo

Radiosensitivity of cloned permanent murine bone marrow stromal cell lines: nonuniform effect of low dose rate. 1988

T J FitzGerald, and P Anklesaria, and D Le, and M A Sakakeeny, and K Kase, and J S Greenberger
Department of Radiation Oncology, University of Massachusetts Medical Center, Worcester 01605.

The x-irradiation biology of supportive stromal cells of the bone marrow microenvironment was investigated by using cloned permanent cell lines that were established from hematopoietically active murine long-term bone marrow cultures. X-irradiation survival curves were derived for each cell line at either 120 cGy/min or clinical low dose rate (LDR) (5 cGy/min) that is used in total body irradiation protocols prior to bone marrow transplantation. Four cell lines, MBA-1, MBA-13, 14F2.1, and D2XRII (Group I) demonstrated a significant increase in D0 at 5 cGy/min (280 cGy, 270 cGy, 210 cGy, and 240 cGy, respectively) compared to 120 cGy/min (215 cGy, 210 cGy, 157 cGy, and 210 cGy, respectively) (p less than 0.05). In contrast, three other clonal cell lines, +/+ #2 cl 4, S1d #3, and GPI alpha-1 (Group II) showed no significant dose rate dependent change in D0 at 5 cGy/min (164 cGy, 174 cGy, and 159 cGy, respectively) compared to 120 cGy/min (159 cGy, 167 cGy, and 143 cGy, respectively). Group I and II cell lines could not be distinguished by differences in synthesis of extracellular matrix proteins including laminin, collagen types I and IV, or histochemically detectable enzymes. Three Group I lines (MBA-1, MBA-13, and D2XRII) and one Group II line, Sld #3, showed decreased support capacity for cocultivated hematopoietic stem cells in vitro. All seven lines had detectable polyA+ mRNA for monocyte colony-stimulating factor (M-CSF) as detected by molecular hybridization; one had detectable levels of polyA+ mRNA for granulocyte-macrophage colony-stimulating factor (GM-CSF) (D2XRII); one, detectable levels of polyA+ mRNA for interleukin 1 (IL-1); and none of the seven had detectable polyA+ mRNA for granulocyte colony-stimulating factor (G-CSF) or IL-3 (multi-CSF). The data indicate that some cells of the hematopoietic microenvironment may not be selectively protected by clinical low dose rate irradiation.(ABSTRACT TRUNCATED AT 250 WORDS)

UI MeSH Term Description Entries
D007797 Laminin Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion. Merosin,Glycoprotein GP-2,Laminin M,Laminin M Chain,Chain, Laminin M,Glycoprotein GP 2,M Chain, Laminin
D011836 Radiation Tolerance The ability of some cells or tissues to survive lethal doses of IONIZING RADIATION. Tolerance depends on the species, cell type, and physical and chemical variables, including RADIATION-PROTECTIVE AGENTS and RADIATION-SENSITIZING AGENTS. Radiation Sensitivity,Radiosensitivity,Sensitivity, Radiation,Tolerance, Radiation,Radiation Sensitivities,Radiation Tolerances,Radiosensitivities,Sensitivities, Radiation,Tolerances, Radiation
D001853 Bone Marrow The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells. Marrow,Red Marrow,Yellow Marrow,Marrow, Bone,Marrow, Red,Marrow, Yellow
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D003238 Connective Tissue Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX. Connective Tissues,Tissue, Connective,Tissues, Connective
D004307 Dose-Response Relationship, Radiation The relationship between the dose of administered radiation and the response of the organism or tissue to the radiation. Dose Response Relationship, Radiation,Dose-Response Relationships, Radiation,Radiation Dose-Response Relationship,Radiation Dose-Response Relationships,Relationship, Radiation Dose-Response,Relationships, Radiation Dose-Response
D005353 Fibronectins Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins. Cold-Insoluble Globulins,LETS Proteins,Fibronectin,Opsonic Glycoprotein,Opsonic alpha(2)SB Glycoprotein,alpha 2-Surface Binding Glycoprotein,Cold Insoluble Globulins,Globulins, Cold-Insoluble,Glycoprotein, Opsonic,Proteins, LETS,alpha 2 Surface Binding Glycoprotein
D006085 Graft Survival The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host. Graft Survivals,Survival, Graft,Survivals, Graft

Related Publications

T J FitzGerald, and P Anklesaria, and D Le, and M A Sakakeeny, and K Kase, and J S Greenberger
Radiosensitivity of permanent human bone marrow stromal cell lines: effect of dose rate.
November 1988, International journal of radiation oncology, biology, physics,
T J FitzGerald, and P Anklesaria, and D Le, and M A Sakakeeny, and K Kase, and J S Greenberger
Proteoglycan synthesis in two murine bone marrow stromal cell lines.
December 1987, Blood,
T J FitzGerald, and P Anklesaria, and D Le, and M A Sakakeeny, and K Kase, and J S Greenberger
Radiosensitivity of human bone marrow granulocyte-macrophage progenitor cells and stromal colony-forming cells: effect of dose rate.
August 1986, Radiation research,
T J FitzGerald, and P Anklesaria, and D Le, and M A Sakakeeny, and K Kase, and J S Greenberger
Reproducible establishment of hemopoietic supportive stromal cell lines from murine bone marrow.
February 1989, Experimental hematology,
T J FitzGerald, and P Anklesaria, and D Le, and M A Sakakeeny, and K Kase, and J S Greenberger
Radiosensitivity, recovery and dose-rate effect in three human glioma cell lines.
September 1990, Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology,
T J FitzGerald, and P Anklesaria, and D Le, and M A Sakakeeny, and K Kase, and J S Greenberger
Cloned stromal cell lines derived from human Whitlock/Witte-type long-term bone marrow cultures.
August 1990, Experimental hematology,
T J FitzGerald, and P Anklesaria, and D Le, and M A Sakakeeny, and K Kase, and J S Greenberger
Constitutive and modulated cytokine expression in two permanent human bone marrow stromal cell lines.
January 1996, Experimental hematology,
T J FitzGerald, and P Anklesaria, and D Le, and M A Sakakeeny, and K Kase, and J S Greenberger
Pulsed low dose-rate irradiation response in isogenic HNSCC cell lines with different radiosensitivity.
March 2020, Radiology and oncology,
T J FitzGerald, and P Anklesaria, and D Le, and M A Sakakeeny, and K Kase, and J S Greenberger
A novel 37-Kd adhesive membrane protein from cloned murine bone marrow stromal cells and cloned murine hematopoietic progenitor cells.
September 1993, Blood,
T J FitzGerald, and P Anklesaria, and D Le, and M A Sakakeeny, and K Kase, and J S Greenberger
Establishment of two permanent human bone marrow stromal cell lines with long-term post irradiation feeder capacity.
April 1994, Blood,
Copied contents to your clipboard!