Biomaterial Database

Structural elucidation of complex carbohydrates. 1988

V N Reinhold, and S Santikarn

Department of Nutrition, Harvard School of Public Health, Boston, Massachusetts 02115.

Many current problems in immunology depend on a detailed understanding of oligosaccharide and glycoconjugate structures. In contrast to other biopolymers, oligosaccharides do not have well established and sensitive analytical procedures for the determination of their structures. Additionally, oligosaccharides because of branching, linkage, and structural isomerism, pose specific complications unique to this biopolymer. We have discussed MS data utilizing DCI and FAB ionization and presented new information that indicates FAB-MS can provide a wealth of structural information including sequence, branching and molecular weight. In the final sections FAB ionization and collision induced dissociation, have been discussed, two adjunct techniques that amplify the analytical value of MS instrumentation in the pursuit of oligosaccharide structure.

UI	MeSH Term	Description	Entries
D007120	Immunochemistry	Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002240	Carbohydrate Sequence	The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.	Carbohydrate Sequences,Sequence, Carbohydrate,Sequences, Carbohydrate
D002241	Carbohydrates	A class of organic compounds composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n. The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES.	Carbohydrate
D013058	Mass Spectrometry	An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.	Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass