Lichen planus: altered AIM2 and NLRP1 expression in skin lesions and defective activation in peripheral blood mononuclear cells. 2019

R Domingues, and A J Pietrobon, and G C Carvalho, and N Z Pereira, and N V Pereira, and M N Sotto, and V Aoki, and A J S Duarte, and M N Sato
Laboratory of Investigation in Medicine, LIM-56, Department of Dermatology, Tropical Medicine Institute of São Paulo, University of São Paulo Medical School, São Paulo, Brazil.

BACKGROUND Lichen planus (LP) is an inflammatory skin disease with unknown aetiology. Activation by pathogen-associated molecular patterns or environmental stimuli may activate some components of inflammasomes that contribute to the inflammatory process in LP lesions. OBJECTIVE To characterize the inflammasomes in skin lesions and peripheral blood mononuclear cells (PBMCs) of patients with LP under Toll-like receptor (TLR) activation. METHODS In total, 15 patients with LP and 14 healthy controls (HCs) were enrolled in the study. Inflammasome expression in skin was evaluated by real-time PCR and immunohistochemistry, while ELISA was used to assess the production of interleukin (IL)-1β by PBMCs under stimulation with TLR4 and TLR7/TLR8 agonists and adenosine triphosphate (ATP). RESULTS Compared with the levels in HC samples, increased expression of the inflammasome AIM2 was verified in both epidermal and dermal sections of LP skin lesions, whereas NLRP1 and IL-β expression levels were enhanced in the dermis. LP skin lesion samples exhibited higher AIM2 transcript levels, similar NLRP1 levels and lower pro-IL-1β mRNA levels compared with HC samples. We verified that, compared with PBMCs from HC subjects, PBMCs from patients with LP produced similar amounts of IL-1β after induction by TLR4 agonists but lower IL-1β levels after induction by TLR7/TLR8 agonists, regardless of the addition of ATP. CONCLUSIONS Alterations in innate immunity, such as inflammasome component expression in skin lesions and PBMCs, were observed in patients with LP. Further investigations of dysfunctional inflammasome activation and the chronic inflammatory status of LP are required.

UI MeSH Term Description Entries
D007113 Immunity, Innate The capacity of a normal organism to remain unaffected by microorganisms and their toxins. It results from the presence of naturally occurring ANTI-INFECTIVE AGENTS, constitutional factors such as BODY TEMPERATURE and immediate acting immune cells such as NATURAL KILLER CELLS. Immunity, Native,Immunity, Natural,Immunity, Non-Specific,Resistance, Natural,Innate Immune Response,Innate Immunity,Immune Response, Innate,Immune Responses, Innate,Immunity, Non Specific,Innate Immune Responses,Native Immunity,Natural Immunity,Natural Resistance,Non-Specific Immunity
D007963 Leukocytes, Mononuclear Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules. Mononuclear Leukocyte,Mononuclear Leukocytes,PBMC Peripheral Blood Mononuclear Cells,Peripheral Blood Human Mononuclear Cells,Peripheral Blood Mononuclear Cell,Peripheral Blood Mononuclear Cells,Leukocyte, Mononuclear
D008010 Lichen Planus An inflammatory, pruritic disease of the skin and mucous membranes, which can be either generalized or localized. It is characterized by distinctive purplish, flat-topped papules having a predilection for the trunk and flexor surfaces. The lesions may be discrete or coalesce to form plaques. Histologically, there is a "saw-tooth" pattern of epidermal hyperplasia and vacuolar alteration of the basal layer of the epidermis along with an intense upper dermal inflammatory infiltrate composed predominantly of T-cells. Etiology is unknown. Cutaneous Lichen Planus,Lichen Planopilaris,Lichen Ruber Planus,Mucosal Lichen Planus,Lichen Rubra Planus,Lichen Planus, Cutaneous,Lichen Planus, Mucosal,Planopilaris, Lichen
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000070576 NLR Proteins Intracellular signaling proteins that are defined by the presence of a NUCLEOTIDE-binding region and LEUCINE-rich repeats. Their general structure consists of any of a variety of effector domains at their N-termini such as a caspase recruitment domain (CARD), a central nucleotide-binding domain, and a variable number of C-terminal leucine-rich repeats. They are important for pathogen recognition in the INNATE IMMUNE RESPONSE of animals and plants. Members of the NLR protein family include the NOD SIGNALING ADAPTOR PROTEINS. NOD-like Receptor,Nucleotide-Binding Domain Leucine-Rich Repeat Protein,NLR Protein,NOD-like Receptors,Nucleotide-binding Domain Leucine-rich Repeat Proteins,NOD like Receptor,NOD like Receptors,Nucleotide Binding Domain Leucine Rich Repeat Protein,Nucleotide binding Domain Leucine rich Repeat Proteins,Protein, NLR,Proteins, NLR,Receptor, NOD-like,Receptors, NOD-like
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults

Related Publications

R Domingues, and A J Pietrobon, and G C Carvalho, and N Z Pereira, and N V Pereira, and M N Sotto, and V Aoki, and A J S Duarte, and M N Sato
June 1978, The British journal of dermatology,
R Domingues, and A J Pietrobon, and G C Carvalho, and N Z Pereira, and N V Pereira, and M N Sotto, and V Aoki, and A J S Duarte, and M N Sato
August 2004, The British journal of dermatology,
R Domingues, and A J Pietrobon, and G C Carvalho, and N Z Pereira, and N V Pereira, and M N Sotto, and V Aoki, and A J S Duarte, and M N Sato
April 2013, Inflammation,
R Domingues, and A J Pietrobon, and G C Carvalho, and N Z Pereira, and N V Pereira, and M N Sotto, and V Aoki, and A J S Duarte, and M N Sato
May 2011, Archives of oral biology,
R Domingues, and A J Pietrobon, and G C Carvalho, and N Z Pereira, and N V Pereira, and M N Sotto, and V Aoki, and A J S Duarte, and M N Sato
January 1984, Dermatologica,
R Domingues, and A J Pietrobon, and G C Carvalho, and N Z Pereira, and N V Pereira, and M N Sotto, and V Aoki, and A J S Duarte, and M N Sato
January 2015, Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology,
R Domingues, and A J Pietrobon, and G C Carvalho, and N Z Pereira, and N V Pereira, and M N Sotto, and V Aoki, and A J S Duarte, and M N Sato
April 2016, Inflammation,
R Domingues, and A J Pietrobon, and G C Carvalho, and N Z Pereira, and N V Pereira, and M N Sotto, and V Aoki, and A J S Duarte, and M N Sato
March 2003, Journal of the European Academy of Dermatology and Venereology : JEADV,
R Domingues, and A J Pietrobon, and G C Carvalho, and N Z Pereira, and N V Pereira, and M N Sotto, and V Aoki, and A J S Duarte, and M N Sato
February 1983, The Journal of dermatology,
R Domingues, and A J Pietrobon, and G C Carvalho, and N Z Pereira, and N V Pereira, and M N Sotto, and V Aoki, and A J S Duarte, and M N Sato
January 1994, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology,
Copied contents to your clipboard!