Molecular cloning and preliminary functional analysis of six RING-between-ring (RBR) genes in grass carp (Ctenopharyngodon idellus). 2019

Lifei Luo, and Denghui Zhu, and Rong Huang, and Lv Xiong, and Rumana Mehjabin, and Libo He, and Lanjie Liao, and Yongming Li, and Zuoyan Zhu, and Yaping Wang
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

Ubiquitination is a post-translational modification of proteins that is widely present in eukaryotic cells. There is increasing evidence that ubiquitinated proteins play crucial roles in the immune response process. In mammals, RING-between-RING (RBR) proteins play a key role in regulating immune signaling as the important E3 ubiquitin ligases during ubiquitination. However, the function of RBR in fish is still unclear. In the present study, six RBR genes (RNF19A, RNF19B, RNF144AA, RNF144AB, RNF144B and RNF217) of grass carp (Ctenopharyngodon idellus) were cloned and characterized. Similar to mammals, all six members of RBR family contained RING, in-between-ring (IBR) and transmembrane (TM) domains. These genes were constitutively expressed in all studied tissues, but the relative expression level differed. Following grass carp reovirus(GCRV) infection, the expression of six RBR genes in liver, gill, spleen and intestine significantly altered. Additionally, their expression in Ctenopharyngodon idellus kidney (CIK) cells was significantly increased after GCRV infection. And deficiency of RNF144B in CIK with small interference RNA (siRNA) up-regulated polyinosinic:polycytidylic acid poly(I:C))-induced inflammatory cytokines production, including IFN-I, TNF-α, IL-6, and transcription factor IRF3, which demonstrated that RNF144B was a negative regulator of inflammatory cytokines. Our results suggested that the RBR might play a vital role in regulating immune signaling and laid the foundation for the further mechanism research of RBR in fishes.

UI MeSH Term Description Entries
D011070 Poly I-C Interferon inducer consisting of a synthetic, mismatched double-stranded RNA. The polymer is made of one strand each of polyinosinic acid and polycytidylic acid. Poly(I-C),Poly(rI).Poly(rC),Polyinosinic-Polycytidylic Acid,Polyinosinic-Polycytidylic Acid (High MW),Polyriboinosinic-Polyribocytidylic Acid,Polyribose Inosin-Cytidil,Inosin-Cytidil, Polyribose,Poly I C,Polyinosinic Polycytidylic Acid,Polyriboinosinic Polyribocytidylic Acid,Polyribose Inosin Cytidil
D012087 Reoviridae A family of unenveloped RNA viruses with cubic symmetry. The twelve genera include ORTHOREOVIRUS; ORBIVIRUS; COLTIVIRUS; ROTAVIRUS; Aquareovirus, Cypovirus, Phytoreovirus, Fijivirus, Seadornavirus, Idnoreovirus, Mycoreovirus, and Oryzavirus. Aquareovirus,Cypovirus,Cytoplasmic Polyhedrosis Viruses,Fijivirus,Idnoreovirus,Mycoreovirus,Oryzavirus,Phytoreovirus,Reoviruses, Aquatic,Respiratory Enteric Orphan Viruses,Seadornavirus,Aquareoviruses,Aquatic Reovirus,Aquatic Reoviruses,Cypoviruses,Cytoplasmic Polyhedrosis Virus,Fijiviruses,Idnoreoviruses,Mycoreoviruses,Oryzaviruses,Phytoreoviruses,Polyhedrosis Virus, Cytoplasmic,Polyhedrosis Viruses, Cytoplasmic,Reovirus, Aquatic,Seadornaviruses
D012088 Reoviridae Infections Infections produced by reoviruses, general or unspecified. Reovirus Infections,Infections, Reoviridae,Infection, Reoviridae,Infection, Reovirus,Infections, Reovirus,Reoviridae Infection,Reovirus Infection
D002347 Carps Common name for a number of different species of fish in the family Cyprinidae. This includes, among others, the common carp, crucian carp, grass carp, and silver carp. Carassius carassius,Crucian Carp,Cyprinus,Grass Carp,Carp,Ctenopharyngodon idellus,Cyprinus carpio,Hypophthalmichthys molitrix,Koi Carp,Silver Carp,Carp, Crucian,Carp, Grass,Carp, Koi,Carp, Silver,Carps, Crucian,Carps, Grass,Carps, Silver,Crucian Carps,Grass Carps,Silver Carps
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D005393 Fish Diseases Diseases of freshwater, marine, hatchery or aquarium fish. This term includes diseases of both teleosts (true fish) and elasmobranchs (sharks, rays and skates). Disease, Fish,Diseases, Fish,Fish Disease
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D016207 Cytokines Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner. Cytokine
D017422 Sequence Analysis, DNA A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis. DNA Sequence Analysis,Sequence Determination, DNA,Analysis, DNA Sequence,DNA Sequence Determination,DNA Sequence Determinations,DNA Sequencing,Determination, DNA Sequence,Determinations, DNA Sequence,Sequence Determinations, DNA,Analyses, DNA Sequence,DNA Sequence Analyses,Sequence Analyses, DNA,Sequencing, DNA

Related Publications

Lifei Luo, and Denghui Zhu, and Rong Huang, and Lv Xiong, and Rumana Mehjabin, and Libo He, and Lanjie Liao, and Yongming Li, and Zuoyan Zhu, and Yaping Wang
January 2022, Fish & shellfish immunology,
Lifei Luo, and Denghui Zhu, and Rong Huang, and Lv Xiong, and Rumana Mehjabin, and Libo He, and Lanjie Liao, and Yongming Li, and Zuoyan Zhu, and Yaping Wang
December 2013, Fish & shellfish immunology,
Lifei Luo, and Denghui Zhu, and Rong Huang, and Lv Xiong, and Rumana Mehjabin, and Libo He, and Lanjie Liao, and Yongming Li, and Zuoyan Zhu, and Yaping Wang
December 2011, Fish & shellfish immunology,
Lifei Luo, and Denghui Zhu, and Rong Huang, and Lv Xiong, and Rumana Mehjabin, and Libo He, and Lanjie Liao, and Yongming Li, and Zuoyan Zhu, and Yaping Wang
June 2013, Fish & shellfish immunology,
Lifei Luo, and Denghui Zhu, and Rong Huang, and Lv Xiong, and Rumana Mehjabin, and Libo He, and Lanjie Liao, and Yongming Li, and Zuoyan Zhu, and Yaping Wang
October 1991, Biochimica et biophysica acta,
Lifei Luo, and Denghui Zhu, and Rong Huang, and Lv Xiong, and Rumana Mehjabin, and Libo He, and Lanjie Liao, and Yongming Li, and Zuoyan Zhu, and Yaping Wang
June 2020, Fish physiology and biochemistry,
Lifei Luo, and Denghui Zhu, and Rong Huang, and Lv Xiong, and Rumana Mehjabin, and Libo He, and Lanjie Liao, and Yongming Li, and Zuoyan Zhu, and Yaping Wang
June 2013, General and comparative endocrinology,
Lifei Luo, and Denghui Zhu, and Rong Huang, and Lv Xiong, and Rumana Mehjabin, and Libo He, and Lanjie Liao, and Yongming Li, and Zuoyan Zhu, and Yaping Wang
January 2014, International journal of genomics,
Lifei Luo, and Denghui Zhu, and Rong Huang, and Lv Xiong, and Rumana Mehjabin, and Libo He, and Lanjie Liao, and Yongming Li, and Zuoyan Zhu, and Yaping Wang
February 2014, Fish physiology and biochemistry,
Lifei Luo, and Denghui Zhu, and Rong Huang, and Lv Xiong, and Rumana Mehjabin, and Libo He, and Lanjie Liao, and Yongming Li, and Zuoyan Zhu, and Yaping Wang
April 2019, Fish & shellfish immunology,
Copied contents to your clipboard!