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Nucleic acid hybridization is extremely important in viral hepatitis research and the newly emerging techniques are now becoming an indispensable resource also in the diagnostic laboratory. This paper provides information on methods currently used for detection of viral nucleic acids with special emphasis on the importance of hepatitis B virus DNA in the serum. Herein, we describe the procedures for preparation and labeling of DNA probes and the principles that regulate dot, slot and Southern blot hybridization. Advantages and shortcomings of filter hybridization are discussed together with the alternative approaches to it. Finally, a collection of laboratory protocols is presented in Appendix #1.
F Bonino, and
E Chiaberge, and
M R Brunetto, and
P Piantino, and
E Maran
August 1990,
Clinical biochemistry,
F Bonino, and
E Chiaberge, and
M R Brunetto, and
P Piantino, and
E Maran
January 1988,
La Ricerca in clinica e in laboratorio,
F Bonino, and
E Chiaberge, and
M R Brunetto, and
P Piantino, and
E Maran
January 1978,
Advances in neurology,
F Bonino, and
E Chiaberge, and
M R Brunetto, and
P Piantino, and
E Maran
September 1985,
Clinics in laboratory medicine,
F Bonino, and
E Chiaberge, and
M R Brunetto, and
P Piantino, and
E Maran
January 1983,
Current topics in microbiology and immunology,
F Bonino, and
E Chiaberge, and
M R Brunetto, and
P Piantino, and
E Maran
July 1988,
Laboratory investigation; a journal of technical methods and pathology,
F Bonino, and
E Chiaberge, and
M R Brunetto, and
P Piantino, and
E Maran
November 2006,
Seminars in liver disease,
F Bonino, and
E Chiaberge, and
M R Brunetto, and
P Piantino, and
E Maran
October 2005,
Nano letters,
F Bonino, and
E Chiaberge, and
M R Brunetto, and
P Piantino, and
E Maran
October 1985,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
F Bonino, and
E Chiaberge, and
M R Brunetto, and
P Piantino, and
E Maran
January 2015,
Jundishapur journal of microbiology,
