Molecular characterization of Ehrlichia canis from naturally infected dogs from the state of Rio de Janeiro. 2019

Renata Lins da Costa, and Patrícia Gonzaga Paulino, and Claudia Bezerra da Silva, and Gabriela Lopes Vivas Vitari, and Maristela Peckle Peixoto, and Ana Paula Martinez de Abreu, and Huarrisson Azevedo Santos, and Carlos Luiz Massard
Department of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, BR 465, km 7, Seropédica, Rio de Janeiro, 23890-000, Brazil. renatalins.costa@yahoo.com.br.

The aim of the present study was to evaluate the genetic diversity of Ehrlichia canis in naturally infected dogs from six mesoregions of Rio de Janeiro state. E. canis was diagnosed with a real-time polymerase chain reaction (qPCR) targeting a 93 base pair (bp) fragment of the 16S rDNA gene. To evaluate the genetic diversity of the parasite, we amplified a positive sample from each mesoregion by distinct conventional PCR assays with targets in the gp19 (414 bp), gp36 (814 bp), and p28 (843 bp) genes. A total of 267 samples were collected from dogs in Rio de Janeiro state. Among the samples analyzed, 42.3% (n = 113/267) were 16S rDNA-qPCR positive. When performing PCR for the gp19 and gp36 genes, 100% (n = 113/113) and 5.3% (n = 6/113) of the samples amplified fragments of 414 bp and 814 bp, respectively. The six PCR-positive samples for the gp36 gene also amplified the p28 gene fragment. The characterization based on the gp19 gene demonstrated that it is highly conserved. In protein analysis (TRP36), all samples showed a tandem repeat protein (TRP) that comprised 11 replicates. Seven high-entropy amino acid sites were distributed throughout the gp36 gene. Eleven high-entropy amino acid sites were found throughout the p28 gene. There is a positive selection pressure in both genes (p ≤ 0.05). Comparing and characterizing an organism are useful for providing important information about the host's immune response and identifying new antigenic targets, as well as essential characteristics for the development of vaccines and new diagnostic tools.

UI MeSH Term Description Entries
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D001938 Brazil A country located on the eastern coast of South America, located between Colombia and Peru, that borders the Atlantic Ocean. It is bordered on the north by Venezuela, Guyana, Suriname, and French Guiana, on the south by Uruguay, and on the west by Argentina. The capital is Brasilia.
D004283 Dog Diseases Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used. Canine Diseases,Canine Disease,Disease, Canine,Disease, Dog,Diseases, Canine,Diseases, Dog,Dog Disease
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D014644 Genetic Variation Genotypic differences observed among individuals in a population. Genetic Diversity,Variation, Genetic,Diversity, Genetic,Diversities, Genetic,Genetic Diversities,Genetic Variations,Variations, Genetic
D016873 Ehrlichiosis A tick-borne disease characterized by FEVER; HEADACHE; myalgias; ANOREXIA; and occasionally RASH. It is caused by several bacterial species and can produce disease in DOGS; CATTLE; SHEEP; GOATS; HORSES; and humans. The primary species causing human disease are EHRLICHIA CHAFFEENSIS; ANAPLASMA PHAGOCYTOPHILUM; and Ehrlichia ewingii. E chaffeensis Infection,E ewingii Infection,E. chaffeensis Infection,E. ewingii Infection,Ehrlichia Infection,Ehrlichia chaffeensis Infection,Ehrlichia ewingii Infection,Human Ehrlichiosis,E chaffeensis Infections,E ewingii Infections,E. ewingii Infections,Ehrlichia Infections,Ehrlichia chaffeensis Infections,Ehrlichia ewingii Infections,Ehrlichioses,Human Ehrlichioses,Infection, E chaffeensis,Infection, E. chaffeensis,Infections, E chaffeensis
D041061 Ehrlichia canis Species of gram-negative bacteria in the family ANAPLASMATACEAE, causing EHRLICHIOSIS in DOGS. The most common vector is the brown dog tick. It can also cause disease in humans.

Related Publications

Renata Lins da Costa, and Patrícia Gonzaga Paulino, and Claudia Bezerra da Silva, and Gabriela Lopes Vivas Vitari, and Maristela Peckle Peixoto, and Ana Paula Martinez de Abreu, and Huarrisson Azevedo Santos, and Carlos Luiz Massard
January 2014, Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria,
Renata Lins da Costa, and Patrícia Gonzaga Paulino, and Claudia Bezerra da Silva, and Gabriela Lopes Vivas Vitari, and Maristela Peckle Peixoto, and Ana Paula Martinez de Abreu, and Huarrisson Azevedo Santos, and Carlos Luiz Massard
January 2005, Veterinary clinical pathology,
Renata Lins da Costa, and Patrícia Gonzaga Paulino, and Claudia Bezerra da Silva, and Gabriela Lopes Vivas Vitari, and Maristela Peckle Peixoto, and Ana Paula Martinez de Abreu, and Huarrisson Azevedo Santos, and Carlos Luiz Massard
December 2021, Acta parasitologica,
Renata Lins da Costa, and Patrícia Gonzaga Paulino, and Claudia Bezerra da Silva, and Gabriela Lopes Vivas Vitari, and Maristela Peckle Peixoto, and Ana Paula Martinez de Abreu, and Huarrisson Azevedo Santos, and Carlos Luiz Massard
November 2023, Scientific reports,
Renata Lins da Costa, and Patrícia Gonzaga Paulino, and Claudia Bezerra da Silva, and Gabriela Lopes Vivas Vitari, and Maristela Peckle Peixoto, and Ana Paula Martinez de Abreu, and Huarrisson Azevedo Santos, and Carlos Luiz Massard
April 2008, Veterinary parasitology,
Renata Lins da Costa, and Patrícia Gonzaga Paulino, and Claudia Bezerra da Silva, and Gabriela Lopes Vivas Vitari, and Maristela Peckle Peixoto, and Ana Paula Martinez de Abreu, and Huarrisson Azevedo Santos, and Carlos Luiz Massard
August 2018, The American journal of tropical medicine and hygiene,
Renata Lins da Costa, and Patrícia Gonzaga Paulino, and Claudia Bezerra da Silva, and Gabriela Lopes Vivas Vitari, and Maristela Peckle Peixoto, and Ana Paula Martinez de Abreu, and Huarrisson Azevedo Santos, and Carlos Luiz Massard
January 2019, Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria,
Renata Lins da Costa, and Patrícia Gonzaga Paulino, and Claudia Bezerra da Silva, and Gabriela Lopes Vivas Vitari, and Maristela Peckle Peixoto, and Ana Paula Martinez de Abreu, and Huarrisson Azevedo Santos, and Carlos Luiz Massard
June 2015, Research in veterinary science,
Renata Lins da Costa, and Patrícia Gonzaga Paulino, and Claudia Bezerra da Silva, and Gabriela Lopes Vivas Vitari, and Maristela Peckle Peixoto, and Ana Paula Martinez de Abreu, and Huarrisson Azevedo Santos, and Carlos Luiz Massard
January 2012, Revista do Instituto de Medicina Tropical de Sao Paulo,
Renata Lins da Costa, and Patrícia Gonzaga Paulino, and Claudia Bezerra da Silva, and Gabriela Lopes Vivas Vitari, and Maristela Peckle Peixoto, and Ana Paula Martinez de Abreu, and Huarrisson Azevedo Santos, and Carlos Luiz Massard
March 2018, Acta veterinaria Hungarica,
Copied contents to your clipboard!