β-Galactosidase from Exiguobacterium acetylicum: Cloning, expression, purification and characterization. 2019

Carla Aburto, and Carlos Castillo, and Fabián Cornejo, and Mauricio Arenas-Salinas, and Claudio Vásquez, and Cecilia Guerrero, and Felipe Arenas, and Andrés Illanes, and Carlos Vera
School of Biochemical Engineering, Pontificia Universidad Católica de Valparaíso (PUCV), Valparaíso, Chile.

The main goal of this work was to evaluate the performance of β-galactosidase from Exiguobacterium acetylicum MF03 in both hydrolysis and transgalactosylation reactions from different substrates. The enzyme gene was expressed in Escherichia coli BL21 (DE3), sequenced, and subjected to bioinformatic and kinetic assessment. Results showed that the enzyme was able to hydrolyze lactulose and o-nitrophenyl-β-d-galactopyranoside, but unable to hydrolyze lactose, o-nitrophenyl-β-d-glucopyranoside, butyl- and pentyl-β-d-galactosides. This unique and novel substrate specificity converts the E. acetylicum MF03 β-galactosidase into an ideal catalyst for the formulation of an enzymatic kit for lactulose quantification in thermally processed milk. This is because costly steps to eliminate glucose (resulting from hydrolysis of lactose when a customary β-galactosidase is used) can be avoided.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006868 Hydrolysis The process of cleaving a chemical compound by the addition of a molecule of water.
D001406 Bacillaceae A family of bacteria which produce endospores. They are mostly saprophytes from soil, but a few are insect or animal parasites or pathogens.
D001616 beta-Galactosidase A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1. Lactases,Dairyaid,Lactaid,Lactogest,Lactrase,beta-D-Galactosidase,beta-Galactosidase A1,beta-Galactosidase A2,beta-Galactosidase A3,beta-Galactosidases,lac Z Protein,Protein, lac Z,beta D Galactosidase,beta Galactosidase,beta Galactosidase A1,beta Galactosidase A2,beta Galactosidase A3,beta Galactosidases
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions
D055162 Biocatalysis The facilitation of biochemical reactions with the aid of naturally occurring catalysts such as ENZYMES.

Related Publications

Carla Aburto, and Carlos Castillo, and Fabián Cornejo, and Mauricio Arenas-Salinas, and Claudio Vásquez, and Cecilia Guerrero, and Felipe Arenas, and Andrés Illanes, and Carlos Vera
July 2023, Molecular biotechnology,
Carla Aburto, and Carlos Castillo, and Fabián Cornejo, and Mauricio Arenas-Salinas, and Claudio Vásquez, and Cecilia Guerrero, and Felipe Arenas, and Andrés Illanes, and Carlos Vera
January 2015, Letters in applied microbiology,
Carla Aburto, and Carlos Castillo, and Fabián Cornejo, and Mauricio Arenas-Salinas, and Claudio Vásquez, and Cecilia Guerrero, and Felipe Arenas, and Andrés Illanes, and Carlos Vera
July 2022, Molecules (Basel, Switzerland),
Carla Aburto, and Carlos Castillo, and Fabián Cornejo, and Mauricio Arenas-Salinas, and Claudio Vásquez, and Cecilia Guerrero, and Felipe Arenas, and Andrés Illanes, and Carlos Vera
February 2011, Applied microbiology and biotechnology,
Carla Aburto, and Carlos Castillo, and Fabián Cornejo, and Mauricio Arenas-Salinas, and Claudio Vásquez, and Cecilia Guerrero, and Felipe Arenas, and Andrés Illanes, and Carlos Vera
January 2016, Journal of microbiology and biotechnology,
Carla Aburto, and Carlos Castillo, and Fabián Cornejo, and Mauricio Arenas-Salinas, and Claudio Vásquez, and Cecilia Guerrero, and Felipe Arenas, and Andrés Illanes, and Carlos Vera
June 2015, Cellular and molecular biology (Noisy-le-Grand, France),
Carla Aburto, and Carlos Castillo, and Fabián Cornejo, and Mauricio Arenas-Salinas, and Claudio Vásquez, and Cecilia Guerrero, and Felipe Arenas, and Andrés Illanes, and Carlos Vera
November 2023, Molecules (Basel, Switzerland),
Carla Aburto, and Carlos Castillo, and Fabián Cornejo, and Mauricio Arenas-Salinas, and Claudio Vásquez, and Cecilia Guerrero, and Felipe Arenas, and Andrés Illanes, and Carlos Vera
January 2013, BMC biotechnology,
Carla Aburto, and Carlos Castillo, and Fabián Cornejo, and Mauricio Arenas-Salinas, and Claudio Vásquez, and Cecilia Guerrero, and Felipe Arenas, and Andrés Illanes, and Carlos Vera
January 1992, Bioscience, biotechnology, and biochemistry,
Carla Aburto, and Carlos Castillo, and Fabián Cornejo, and Mauricio Arenas-Salinas, and Claudio Vásquez, and Cecilia Guerrero, and Felipe Arenas, and Andrés Illanes, and Carlos Vera
December 2020, Microorganisms,
Copied contents to your clipboard!