OsDCL3b affects grain yield and quality in rice. 2019

Peng-Fei Liao, and Jie-Xiu Ouyang, and Jian-Jun Zhang, and Lan Yang, and Xin Wang, and Xiao-Jue Peng, and Dong Wang, and You-Lin Zhu, and Shao-Bo Li
Key Laboratory of Molecular Biology and Gene Engineering in Jiangxi Province, School of Life Sciences, Nanchang University, Nanchang, 330031, China.

We reported that knockdown of OsDCL3b decreased grain yield but increased grain quality in rice, which is helpful for molecular breeding in crops. Multiple DICER-LIKE (DCL) genes usually exist and show diverse biochemical and phenotypic functions in land plants. In rice, the biochemical function of OsDCL3b is known to process 24-nucleotide panicle phased small RNAs, however, its phenotypic functions are unclear. Here we reported that knockdown of OsDCL3b led to reduced pollen fertility, seed setting rate, and decreased grain yield but increased grain quality in rice. To reveal the molecular mechanism of the above phenomena, extracted RNAs from rice panicles of the wild type (WT) and OsDCL3b-RNAi line S6-1 were analyzed by deep sequencing. It showed that knockdown of OsDCL3b affected the biogenesis of both 21- and 24-nucleotide small RNAs including miRNAs and phased small RNAs. Using RNA-seq, 644 up- and 530 down-regulated mRNA genes were identified in panicles of line S6-1, and 550 and 273 differentially spliced genes with various alternative splicing (AS) events were observed in panicles of line S6-1 and WT, respectively, suggesting that OsDCL3b involved in influencing the transcript levels of mRNA genes and the AS events in rice panicles. Thus, our results show that knockdown of OsDCL3b will affect the biogenesis of small RNAs, which is involved in regulating the transcription of mRNA genes, and consequently influence the grain yield and quality in rice.

UI MeSH Term Description Entries
D010641 Phenotype The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment. Phenotypes
D010940 Plant Proteins Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which PLANT PROTEINS, DIETARY is available. Plant Protein,Protein, Plant,Proteins, Plant
D002523 Edible Grain SEEDS used as a major nutritional source, most often the grain from the POACEAE family. Cereals,Cereal Grain,Cereal,Cereal Grains,Edible Grains,Grain, Cereal,Grain, Edible,Grains, Cereal,Grains, Edible
D005298 Fertility The capacity to conceive or to induce conception. It may refer to either the male or female. Fecundity,Below Replacement Fertility,Differential Fertility,Fecundability,Fertility Determinants,Fertility Incentives,Fertility Preferences,Fertility, Below Replacement,Marital Fertility,Natural Fertility,Subfecundity,World Fertility Survey,Determinant, Fertility,Determinants, Fertility,Fertility Determinant,Fertility Incentive,Fertility Preference,Fertility Survey, World,Fertility Surveys, World,Fertility, Differential,Fertility, Marital,Fertility, Natural,Preference, Fertility,Preferences, Fertility,Survey, World Fertility,Surveys, World Fertility,World Fertility Surveys
D012275 Oryza A genus of grass family (Poaceae) that include several rice species. Oryza sativa,Rice,Rices
D012639 Seeds The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield. Diaspores,Elaiosomes,Embryos, Plant,Plant Embryos,Plant Zygotes,Zygotes, Plant,Diaspore,Elaiosome,Embryo, Plant,Plant Embryo,Plant Zygote,Seed,Zygote, Plant
D015536 Down-Regulation A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins. Receptor Down-Regulation,Down-Regulation (Physiology),Downregulation,Down Regulation,Down-Regulation, Receptor
D017343 Genes, Plant The functional hereditary units of PLANTS. Plant Genes,Gene, Plant,Plant Gene
D055785 Gene Knockdown Techniques The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES. Gene Knock Down Techniques,Gene Knock Down,Gene Knock-Down,Gene Knock-Down Techniques,Gene Knockdown,Gene Knock Downs,Gene Knock-Down Technique,Gene Knock-Downs,Gene Knockdown Technique,Gene Knockdowns,Knock Down, Gene,Knock Downs, Gene,Knock-Down Technique, Gene,Knock-Down Techniques, Gene,Knock-Down, Gene,Knock-Downs, Gene,Knockdown Technique, Gene,Knockdown Techniques, Gene,Knockdown, Gene,Knockdowns, Gene,Technique, Gene Knock-Down,Technique, Gene Knockdown,Techniques, Gene Knock-Down,Techniques, Gene Knockdown
D059014 High-Throughput Nucleotide Sequencing Techniques of nucleotide sequence analysis that increase the range, complexity, sensitivity, and accuracy of results by greatly increasing the scale of operations and thus the number of nucleotides, and the number of copies of each nucleotide sequenced. The sequencing may be done by analysis of the synthesis or ligation products, hybridization to preexisting sequences, etc. High-Throughput Sequencing,Illumina Sequencing,Ion Proton Sequencing,Ion Torrent Sequencing,Next-Generation Sequencing,Deep Sequencing,High-Throughput DNA Sequencing,High-Throughput RNA Sequencing,Massively-Parallel Sequencing,Pyrosequencing,DNA Sequencing, High-Throughput,High Throughput DNA Sequencing,High Throughput Nucleotide Sequencing,High Throughput RNA Sequencing,High Throughput Sequencing,Massively Parallel Sequencing,Next Generation Sequencing,Nucleotide Sequencing, High-Throughput,RNA Sequencing, High-Throughput,Sequencing, Deep,Sequencing, High-Throughput,Sequencing, High-Throughput DNA,Sequencing, High-Throughput Nucleotide,Sequencing, High-Throughput RNA,Sequencing, Illumina,Sequencing, Ion Proton,Sequencing, Ion Torrent,Sequencing, Massively-Parallel,Sequencing, Next-Generation

Related Publications

Peng-Fei Liao, and Jie-Xiu Ouyang, and Jian-Jun Zhang, and Lan Yang, and Xin Wang, and Xiao-Jue Peng, and Dong Wang, and You-Lin Zhu, and Shao-Bo Li
February 2020, Science China. Life sciences,
Peng-Fei Liao, and Jie-Xiu Ouyang, and Jian-Jun Zhang, and Lan Yang, and Xin Wang, and Xiao-Jue Peng, and Dong Wang, and You-Lin Zhu, and Shao-Bo Li
January 2016, PloS one,
Peng-Fei Liao, and Jie-Xiu Ouyang, and Jian-Jun Zhang, and Lan Yang, and Xin Wang, and Xiao-Jue Peng, and Dong Wang, and You-Lin Zhu, and Shao-Bo Li
March 2023, Physiology and molecular biology of plants : an international journal of functional plant biology,
Peng-Fei Liao, and Jie-Xiu Ouyang, and Jian-Jun Zhang, and Lan Yang, and Xin Wang, and Xiao-Jue Peng, and Dong Wang, and You-Lin Zhu, and Shao-Bo Li
March 2017, Pest management science,
Peng-Fei Liao, and Jie-Xiu Ouyang, and Jian-Jun Zhang, and Lan Yang, and Xin Wang, and Xiao-Jue Peng, and Dong Wang, and You-Lin Zhu, and Shao-Bo Li
November 2019, The Plant journal : for cell and molecular biology,
Peng-Fei Liao, and Jie-Xiu Ouyang, and Jian-Jun Zhang, and Lan Yang, and Xin Wang, and Xiao-Jue Peng, and Dong Wang, and You-Lin Zhu, and Shao-Bo Li
March 2016, Journal of experimental botany,
Peng-Fei Liao, and Jie-Xiu Ouyang, and Jian-Jun Zhang, and Lan Yang, and Xin Wang, and Xiao-Jue Peng, and Dong Wang, and You-Lin Zhu, and Shao-Bo Li
January 2021, Frontiers in plant science,
Peng-Fei Liao, and Jie-Xiu Ouyang, and Jian-Jun Zhang, and Lan Yang, and Xin Wang, and Xiao-Jue Peng, and Dong Wang, and You-Lin Zhu, and Shao-Bo Li
April 2024, International journal of molecular sciences,
Peng-Fei Liao, and Jie-Xiu Ouyang, and Jian-Jun Zhang, and Lan Yang, and Xin Wang, and Xiao-Jue Peng, and Dong Wang, and You-Lin Zhu, and Shao-Bo Li
May 2024, Plants (Basel, Switzerland),
Peng-Fei Liao, and Jie-Xiu Ouyang, and Jian-Jun Zhang, and Lan Yang, and Xin Wang, and Xiao-Jue Peng, and Dong Wang, and You-Lin Zhu, and Shao-Bo Li
May 2022, Journal of genetics and genomics = Yi chuan xue bao,
Copied contents to your clipboard!