Antiviral Activity of Synthetic Peptides Derived from Physiological Proteins. 2018

Arianna Sala, and Andrea Ardizzoni, and Tecla Ciociola, and Walter Magliani, and Stefania Conti, and Elisabetta Blasi, and Claudio Cermelli
Department of Diagnostic, Clinic and Public Health Medicine, University of Modena and Reggio Emilia, Modena, Italy.

OBJECTIVE New antivirals are needed to supplement or replace currently used drugs. The aim of this study was to evaluate the antiviral activity of synthetic peptides derived from physiological proteins. METHODS Vero cell monolayers were infected with herpes simplex virus 1, vesicular stomatitis virus, adenovirus, and coxsackievirus B5 strains in the presence of different concentrations of the selected peptides and viral yield was determined by plaque reduction assays to evaluate the antiviral activity of the peptides. Virucidal activity was evaluated by determining the residual infectivity of viral suspensions treated for 1 h with the peptides at the same concentrations as in the viral yield assays. RESULTS Among the investigated peptides, the killer peptide proved to exert a considerable antiviral activity against herpes simplex virus, attributable to a direct effect on virus particles, while its derivative K10S showed to be effective against the four investigated virus strains only at the highest concentration tested, yet, the inhibitory effects were only partial. CONCLUSIONS Overall, initial evidence is provided on the antiviral activity of several peptides, as well as of their derivatives. Further investigation is warranted to ascertain the mechanism of action in order to develop new potential antiviral drugs.

UI MeSH Term Description Entries
D010455 Peptides Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS. Peptide,Polypeptide,Polypeptides
D010948 Viral Plaque Assay Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE. Bacteriophage Plaque Assay,Assay, Bacteriophage Plaque,Assay, Viral Plaque,Assays, Bacteriophage Plaque,Assays, Viral Plaque,Bacteriophage Plaque Assays,Plaque Assay, Bacteriophage,Plaque Assay, Viral,Plaque Assays, Bacteriophage,Plaque Assays, Viral,Viral Plaque Assays
D002522 Chlorocebus aethiops A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research. African Green Monkey,Cercopithecus aethiops,Cercopithecus griseoviridis,Cercopithecus griseus,Cercopithecus pygerythrus,Cercopithecus sabeus,Cercopithecus tantalus,Chlorocebus cynosuros,Chlorocebus cynosurus,Chlorocebus pygerythrus,Green Monkey,Grivet Monkey,Lasiopyga weidholzi,Malbrouck,Malbrouck Monkey,Monkey, African Green,Monkey, Green,Monkey, Grivet,Monkey, Vervet,Savanah Monkey,Vervet Monkey,Savannah Monkey,African Green Monkey,Chlorocebus cynosuro,Green Monkey, African,Green Monkeys,Grivet Monkeys,Malbrouck Monkeys,Malbroucks,Monkey, Malbrouck,Monkey, Savanah,Monkey, Savannah,Savannah Monkeys,Vervet Monkeys
D000256 Adenoviridae A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases. Adenoviruses,Ichtadenovirus,Adenovirus,Ichtadenoviruses
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000998 Antiviral Agents Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly. Antiviral,Antiviral Agent,Antiviral Drug,Antivirals,Antiviral Drugs,Agent, Antiviral,Agents, Antiviral,Drug, Antiviral,Drugs, Antiviral
D014709 Vero Cells A CELL LINE derived from the kidney of the African green (vervet) monkey, (CHLOROCEBUS AETHIOPS) used primarily in virus replication studies and plaque assays. Cell, Vero,Cells, Vero,Vero Cell
D050296 Microbial Viability Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate. Bacterial Viability,Virus Viability,Bacteria Viability,Microbial Inactivation,Inactivation, Microbial,Viability, Bacteria,Viability, Bacterial,Viability, Microbial,Viability, Virus
D018116 Vesiculovirus A genus of the family RHABDOVIRIDAE that infects a wide range of vertebrates and invertebrates. The type species is VESICULAR STOMATITIS INDIANA VIRUS. Chandipura virus,Vesicular stomatitis Alagoas virus,Vesiculoviruses
D018259 Herpesvirus 1, Human The type species of SIMPLEXVIRUS causing most forms of non-genital herpes simplex in humans. Primary infection occurs mainly in infants and young children and then the virus becomes latent in the dorsal root ganglion. It then is periodically reactivated throughout life causing mostly benign conditions. HSV-1,Herpes Simplex Virus 1,HHV-1,Herpes Simplex Virus Type 1,Herpesvirus 1 (alpha), Human,Human Herpesvirus 1

Related Publications

Arianna Sala, and Andrea Ardizzoni, and Tecla Ciociola, and Walter Magliani, and Stefania Conti, and Elisabetta Blasi, and Claudio Cermelli
August 2006, Experimental parasitology,
Arianna Sala, and Andrea Ardizzoni, and Tecla Ciociola, and Walter Magliani, and Stefania Conti, and Elisabetta Blasi, and Claudio Cermelli
May 2005, Biochemical and biophysical research communications,
Arianna Sala, and Andrea Ardizzoni, and Tecla Ciociola, and Walter Magliani, and Stefania Conti, and Elisabetta Blasi, and Claudio Cermelli
January 2014, Future microbiology,
Arianna Sala, and Andrea Ardizzoni, and Tecla Ciociola, and Walter Magliani, and Stefania Conti, and Elisabetta Blasi, and Claudio Cermelli
January 2024, Antibiotics (Basel, Switzerland),
Arianna Sala, and Andrea Ardizzoni, and Tecla Ciociola, and Walter Magliani, and Stefania Conti, and Elisabetta Blasi, and Claudio Cermelli
February 2024, International journal of molecular sciences,
Arianna Sala, and Andrea Ardizzoni, and Tecla Ciociola, and Walter Magliani, and Stefania Conti, and Elisabetta Blasi, and Claudio Cermelli
July 2012, Amino acids,
Arianna Sala, and Andrea Ardizzoni, and Tecla Ciociola, and Walter Magliani, and Stefania Conti, and Elisabetta Blasi, and Claudio Cermelli
January 1995, Die Nahrung,
Arianna Sala, and Andrea Ardizzoni, and Tecla Ciociola, and Walter Magliani, and Stefania Conti, and Elisabetta Blasi, and Claudio Cermelli
June 2013, Biochimica et biophysica acta,
Arianna Sala, and Andrea Ardizzoni, and Tecla Ciociola, and Walter Magliani, and Stefania Conti, and Elisabetta Blasi, and Claudio Cermelli
January 2008, Developmental and comparative immunology,
Arianna Sala, and Andrea Ardizzoni, and Tecla Ciociola, and Walter Magliani, and Stefania Conti, and Elisabetta Blasi, and Claudio Cermelli
January 2017, Protein and peptide letters,
Copied contents to your clipboard!