Six strains of Trichoderma spp. and Piriformospora indica have been studied for cadmium tolerance and bioaccumulation capacities by the poisoned food technique. A quantitative assay for Trichoderma spp. and P. indica was conducted in broth cultures supplemented with different cadmium concentrations (0-500 mg/l). In addition, the growth pattern of P. indica was determined by growing the fungus in a solid medium amended with eight concentrations of cadmium (0-200 mg/l). Generally, an increasing cadmium gradient in the culture medium suppressed the ability of fungi for cadmium accumulation. However, a negative relation was observed between the biomass production of fungi and cadmium uptake (q: mg/g biomass). Results showed that Trichoderma spp., especially T. simmonsii [UTFC 10063], are tolerant to cadmium toxicity and have a high ability to cadmium bioaccumulation. The biomass production of T. simmonsii [UTFC 10063] was significantly stimulated and increased by 46.1% when cadmium concentration increased from 0 to 125 mg/l. Moreover, 91.7 and 31.2% of cadmium removal was observed at 10 and 500 mg/l of the cadmium concentration, respectively. P. indica, however, showed a lower tolerance and removal efficiency for cadmium as compared with Trichoderma spp. Therefore, Trichoderma spp., especially T. simmonsii [UTFC 10063], can be exploited as potent bio-removal agents in cadmium-polluted aqueous solutions. Graphical abstract.