BIOMAT Database Logo BIOMAT Database Logo

[Continuous production of anti-hepatitis B surface antigen monoclonal antibody in hollow fiber perfusion bioreactor]. 1988

M H Meng, and G C Chen, and J H Hsieh, and Y Yueh, and S C Chen, and T H Chang, and D Tsao
Development Center for Biotechnology, Taipei, Taiwan, ROC.

Hybridoma cell line H440Ac was cultivated continuously in hollow fiber perfusion bioreactor for fifty days to produce anti-hepatitis B surface antigen (anti-HBsAg) monoclonal antibody. A total of 90 mg anti-HBsAg antibody was produced in the hollow fiber bioreactor with 1000 cm2 membrane surface area. The collected production medium has an average antibody concentration of 0.45 mg/ml with a high concentration of 2.3 mg/ml. This compares with 0.016 and 1.1 mg/ml antibody concentration as collected from spinner flask and mouse ascites. The purity of the produced antibody is 0.085, 0.0028 and 0.026 mg antibody per mg of total harvested protein in the culture media produced from the hollow fiber bioreactor, spinner flasks and mouse ascites, respectively.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D010477 Perfusion Treatment process involving the injection of fluid into an organ or tissue. Perfusions
D002452 Cell Count The number of CELLS of a specific kind, usually measured per unit volume or area of sample. Cell Density,Cell Number,Cell Counts,Cell Densities,Cell Numbers,Count, Cell,Counts, Cell,Densities, Cell,Density, Cell,Number, Cell,Numbers, Cell
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D003584 Cytological Techniques Methods used to study CELLS. Cytologic Technics,Cytological Technic,Cytological Technics,Cytological Technique,Technic, Cytological,Technics, Cytological,Technique, Cytological,Techniques, Cytological,Cytologic Technic,Technic, Cytologic,Technics, Cytologic
D004867 Equipment Design Methods and patterns of fabricating machines and related hardware. Design, Equipment,Device Design,Medical Device Design,Design, Medical Device,Designs, Medical Device,Device Design, Medical,Device Designs, Medical,Medical Device Designs,Design, Device,Designs, Device,Designs, Equipment,Device Designs,Equipment Designs

Related Publications

M H Meng, and G C Chen, and J H Hsieh, and Y Yueh, and S C Chen, and T H Chang, and D Tsao
Production of monoclonal antibody against hepatitis B surface antigen.
January 1995, Journal of the Medical Association of Thailand = Chotmaihet thangphaet,
M H Meng, and G C Chen, and J H Hsieh, and Y Yueh, and S C Chen, and T H Chang, and D Tsao
Continuous hybridoma growth and monoclonal antibody production in hollow fiber reactors-separators.
May 1986, Biotechnology and bioengineering,
M H Meng, and G C Chen, and J H Hsieh, and Y Yueh, and S C Chen, and T H Chang, and D Tsao
Enhancement of antibody production to hepatitis B surface antigen by anti-idiotypic antibody.
September 1986, Gut,
M H Meng, and G C Chen, and J H Hsieh, and Y Yueh, and S C Chen, and T H Chang, and D Tsao
Economic comparison of diagnostic antibody production in perfusion stirred tank and in hollow fiber bioreactor processes.
January 2011, Biotechnology progress,
M H Meng, and G C Chen, and J H Hsieh, and Y Yueh, and S C Chen, and T H Chang, and D Tsao
Characterization of anti-hepatitis B surface antigen monoclonal antibodies.
January 1983, Intervirology,
M H Meng, and G C Chen, and J H Hsieh, and Y Yueh, and S C Chen, and T H Chang, and D Tsao
Human monoclonal antibody against Hepatitis B virus surface antigen (HBsAg).
August 2007, Antiviral research,
M H Meng, and G C Chen, and J H Hsieh, and Y Yueh, and S C Chen, and T H Chang, and D Tsao
Continuous production of monoclonal antibody in a packed-bed bioreactor.
June 2005, Biotechnology and applied biochemistry,
M H Meng, and G C Chen, and J H Hsieh, and Y Yueh, and S C Chen, and T H Chang, and D Tsao
A human monoclonal IgG1 lambda anti-hepatitis B surface antibody. Production, properties, and applications.
September 1985, Scandinavian journal of immunology,
M H Meng, and G C Chen, and J H Hsieh, and Y Yueh, and S C Chen, and T H Chang, and D Tsao
Continuous rhamnolipid production using denitrifying Pseudomonas aeruginosa cells in hollow-fiber bioreactor.
January 2013, Biotechnology progress,
M H Meng, and G C Chen, and J H Hsieh, and Y Yueh, and S C Chen, and T H Chang, and D Tsao
Monoclonal antibodies to hepatitis B surface antigen: production and characterization.
January 1986, Folia biologica,
Copied contents to your clipboard!