BIOMAT Database Logo BIOMAT Database Logo

Effective genetic markers for identifying the Escherichia coli F4ac receptor status of pigs. 2019

D Hu, and A Rampoldi, and A Bratus-Neuenschwander, and A Hofer, and H U Bertschinger, and P Vögeli, and S Neuenschwander
Institute of Agricultural Sciences, ETH Zurich, Tannenstrasse 1, 8092, Zurich, Switzerland.

The F4ac receptor locus (F4acR), which encodes susceptibility or resistance to Escherichia coli diarrhoea, is inherited as an autosomal recessive monogenetic trait. F4acR is localized on pig chromosome 13 (SSC13q41-q44) near the MUC13 gene. Two flanking markers (CHCF1 and ALGA0106330) with a high linkage disequilibrium (LD) with F4acR were found to be effective for the genetic identification of F4ac-resistant pigs in the Swiss Large White breed (one recombinant out of 2034 genotyped pigs). Three recombinant boars, one each from the Duroc, Swiss Landrace and Piétrain breeds, were genotyped with seven different markers and phenotyped by means of a microscopic adhesion test. Only ALGA0072075, CHCF1 and CHCF3 indicated the correct phenotype. To test the effect of the resistance allele on production traits, 530 Large White pigs from the national test station were investigated. A significant difference existed among the F4acR locus genotypes in the intramuscular fat content of the longissimus dorsi muscle, whereas no other production traits were influenced by the resistance allele. The frequency of the CHCF1-C and ALGA0106330-A alleles associated with resistance in the Swiss Large White population was 60%, which is advantageous for implementing this trait in a breeding programme to select for E. coli F4ac-resistant animals. The selection of resistant pigs should start on the male side due to the inability of resistant sows to produce sufficient amounts of protecting antibodies in the colostrum. Selection of genetically F4ac-resistant pigs is a sustainable and suitable alternative to decreasing animal loss and antibiotic use due to diarrhoea.

UI MeSH Term Description Entries
D008297 Male Males
D003967 Diarrhea An increased liquidity or decreased consistency of FECES, such as running stool. Fecal consistency is related to the ratio of water-holding capacity of insoluble solids to total water, rather than the amount of water present. Diarrhea is not hyperdefecation or increased fecal weight. Diarrheas
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D004927 Escherichia coli Infections Infections with bacteria of the species ESCHERICHIA COLI. E coli Infections,E. coli Infection,Infections, E coli,Infections, Escherichia coli,E coli Infection,E. coli Infections,Escherichia coli Infection,Infection, E coli,Infection, E. coli,Infection, Escherichia coli
D005260 Female Females
D005819 Genetic Markers A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event. Chromosome Markers,DNA Markers,Markers, DNA,Markers, Genetic,Genetic Marker,Marker, Genetic,Chromosome Marker,DNA Marker,Marker, Chromosome,Marker, DNA,Markers, Chromosome
D005838 Genotype The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS. Genogroup,Genogroups,Genotypes
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001422 Bacterial Adhesion Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity. Adhesion, Bacterial,Adhesions, Bacterial,Bacterial Adhesions
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog

Related Publications

D Hu, and A Rampoldi, and A Bratus-Neuenschwander, and A Hofer, and H U Bertschinger, and P Vögeli, and S Neuenschwander
Gene test to elucidate the ETEC F4ab/F4ac receptor status in pigs.
February 2013, Veterinary microbiology,
D Hu, and A Rampoldi, and A Bratus-Neuenschwander, and A Hofer, and H U Bertschinger, and P Vögeli, and S Neuenschwander
Susceptibility towards enterotoxigenic Escherichia coli F4ac diarrhea is governed by the MUC13 gene in pigs.
January 2012, PloS one,
D Hu, and A Rampoldi, and A Bratus-Neuenschwander, and A Hofer, and H U Bertschinger, and P Vögeli, and S Neuenschwander
Refined candidate region specified by haplotype sharing for Escherichia coli F4ab/F4ac susceptibility alleles in pigs.
February 2010, Animal genetics,
D Hu, and A Rampoldi, and A Bratus-Neuenschwander, and A Hofer, and H U Bertschinger, and P Vögeli, and S Neuenschwander
Fine-mapping of the intestinal receptor locus for enterotoxigenic Escherichia coli F4ac on porcine chromosome 13.
December 2002, Animal genetics,
D Hu, and A Rampoldi, and A Bratus-Neuenschwander, and A Hofer, and H U Bertschinger, and P Vögeli, and S Neuenschwander
Refined localization of the Escherichia coli F4ab/F4ac receptor locus on pig chromosome 13.
October 2009, Animal genetics,
D Hu, and A Rampoldi, and A Bratus-Neuenschwander, and A Hofer, and H U Bertschinger, and P Vögeli, and S Neuenschwander
Refined mapping of the Escherichia coli F4ab/F4ac receptor gene(s) on pig chromosome 13.
October 2011, Animal genetics,
D Hu, and A Rampoldi, and A Bratus-Neuenschwander, and A Hofer, and H U Bertschinger, and P Vögeli, and S Neuenschwander
The receptor locus for Escherichia coli F4ab/F4ac in the pig maps distal to the MUC4-LMLN region.
February 2011, Mammalian genome : official journal of the International Mammalian Genome Society,
D Hu, and A Rampoldi, and A Bratus-Neuenschwander, and A Hofer, and H U Bertschinger, and P Vögeli, and S Neuenschwander
Histopathological features in the small intestine of pigs infected with F4ac+ non-enterotoxigenic or enterotoxigenic strains of Escherichia coli.
January 1995, Journal of comparative pathology,
D Hu, and A Rampoldi, and A Bratus-Neuenschwander, and A Hofer, and H U Bertschinger, and P Vögeli, and S Neuenschwander
Experimental infection with Escherichia coli O149:F4ac in weaned piglets.
June 2006, Veterinary microbiology,
D Hu, and A Rampoldi, and A Bratus-Neuenschwander, and A Hofer, and H U Bertschinger, and P Vögeli, and S Neuenschwander
Comparison of the contributions of heat-labile enterotoxin and heat-stable enterotoxin b to the virulence of enterotoxigenic Escherichia coli in F4ac receptor-positive young pigs.
July 2008, Infection and immunity,
Copied contents to your clipboard!