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All disulfide analogs (types A, B and C) of porcine or human endothelin, a 21-amino acid peptide having two intramolecular disulfide bonds, were synthesized and their retention times on HPLC were compared with that of natural endothelin. One of the analogs (type A) having disulfide bonds between positions 1 and 15 and between 3 and 11 was found to be identical with natural endothelin. Random oxidation of fully reduced endothelin formed a mixture of type A and B in a ratio of 3:1, with almost none of type C, which has disulfide bonds between positions 1 and 3 and between 11 and 15. Type A endothelin was also synthesized by the segment condensation procedure in solution applying our maximum protection strategy. This product was found to have full vasoconstricting activity in rat pulmonary artery ring preparations; the potency was as high as that of the natural product.
S Kumagaye, and
H Kuroda, and
K Nakajima, and
T X Watanabe, and
T Kimura, and
T Masaki, and
S Sakakibara
September 1988,
Proceedings of the National Academy of Sciences of the United States of America,
S Kumagaye, and
H Kuroda, and
K Nakajima, and
T X Watanabe, and
T Kimura, and
T Masaki, and
S Sakakibara
November 1994,
Journal of reconstructive microsurgery,
S Kumagaye, and
H Kuroda, and
K Nakajima, and
T X Watanabe, and
T Kimura, and
T Masaki, and
S Sakakibara
January 1989,
Basic research in cardiology,
S Kumagaye, and
H Kuroda, and
K Nakajima, and
T X Watanabe, and
T Kimura, and
T Masaki, and
S Sakakibara
September 1989,
Nihon rinsho. Japanese journal of clinical medicine,
S Kumagaye, and
H Kuroda, and
K Nakajima, and
T X Watanabe, and
T Kimura, and
T Masaki, and
S Sakakibara
November 1989,
Neurologia medico-chirurgica,
S Kumagaye, and
H Kuroda, and
K Nakajima, and
T X Watanabe, and
T Kimura, and
T Masaki, and
S Sakakibara
April 1988,
FEBS letters,
S Kumagaye, and
H Kuroda, and
K Nakajima, and
T X Watanabe, and
T Kimura, and
T Masaki, and
S Sakakibara
May 2010,
Pflugers Archiv : European journal of physiology,
S Kumagaye, and
H Kuroda, and
K Nakajima, and
T X Watanabe, and
T Kimura, and
T Masaki, and
S Sakakibara
October 1999,
Zhongguo yao li xue bao = Acta pharmacologica Sinica,
S Kumagaye, and
H Kuroda, and
K Nakajima, and
T X Watanabe, and
T Kimura, and
T Masaki, and
S Sakakibara
November 1988,
Pflugers Archiv : European journal of physiology,
S Kumagaye, and
H Kuroda, and
K Nakajima, and
T X Watanabe, and
T Kimura, and
T Masaki, and
S Sakakibara
December 1988,
British journal of pharmacology,
