Biomaterial Database

A catechol biosensor based on immobilizing laccase to Fe3O4@Au core-shell nanoparticles. 2019

Changiz Karami, and Mohammad Ali Taher

Department of Chemistry, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran. Electronic address: changiz.karami@gmail.com.

This work is directed towards the synthesis of the magnetic nanoparticle in a core and a gold shell with immobilized of laccase on its surface (Fe3O4@Au@ Lac). The prepared Fe3O4@Au@ Lac core/shell nanoparticles are characterized by means TEM, SEM, DLS, zeta potential, UV-Vis, and TGA. Meanwhile, as an example of the applications, Biosensor activity was investigated by using the oxidation of catechol and recording the UV-Vis absorption in the 402 nm wavelength. The biosensor also demonstrated optimum activity at pH 5.0, reaction time at 40 min, and 35 mg the amount of biosensor. Linear response in the catechol concentration range of 5.0-70.0 μM. The limit of detection and the apparent Michaels-Menten constant (Km) of the biosensor were 2 μM and 15 μM respectively.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D002396	Catechols	A group of 1,2-benzenediols that contain the general formula R-C6H5O2.	Pyrocatechols,o-Dihydroxybenzenes,ortho-Dihydroxybenzenes,o Dihydroxybenzenes,ortho Dihydroxybenzenes
D004789	Enzyme Activation	Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.	Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D004800	Enzymes, Immobilized	Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.	Immobilized Enzymes,Enzyme, Immobilized,Immobilized Enzyme
D006046	Gold	A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity
D015374	Biosensing Techniques	Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.	Bioprobes,Biosensors,Electrodes, Enzyme,Biosensing Technics,Bioprobe,Biosensing Technic,Biosensing Technique,Biosensor,Electrode, Enzyme,Enzyme Electrode,Enzyme Electrodes,Technic, Biosensing,Technics, Biosensing,Technique, Biosensing,Techniques, Biosensing
D042845	Laccase	A copper-containing oxidoreductase enzyme that catalyzes the oxidation of 4-benzenediol to 4-benzosemiquinone. It also has activity towards a variety of O-quinols and P-quinols. It primarily found in FUNGI and is involved in LIGNIN degradation, pigment biosynthesis and detoxification of lignin-derived products.	Laccase B,Laccase I,Laccase II,Laccase III,Urishiol Oxidase,p-Diphenol Oxidase,Oxidase, Urishiol,Oxidase, p-Diphenol,p Diphenol Oxidase
D052203	Ferrosoferric Oxide	Iron (II,III) oxide (Fe3O4). It is a black ore of IRON that forms opaque crystals and exerts strong magnetism.	Ferumoxytol,Feraheme,Ferriferrous Oxide,Magnetite,Oxide, Ferriferrous,Oxide, Ferrosoferric
D053761	Nanocomposites	Nanometer-scale composite structures composed of organic molecules intimately incorporated with inorganic molecules. (Glossary of Biotechnology and Nanobiotechology Terms, 4th ed)	Nanocomposite