Mature zygotic embryos from three seed sources of loblolly pine were cultured on callus induction medium containing 10 mg l-1 α-naphthaleneacetic acid, 4 mg l-1 benzyladenine (BA), 400 mg l-1 casein hydrolysate, and 400 mg l-1 glutamine for 6 weeks. Light-yellow, loose, glossy, globular callus was formed, and the highest frequency was 35.7%. The highest differentiation frequency of callus on adventitious bud induction medium was 62.1%. After culture of calli with adventitious buds on elongation medium for 6 weeks, adventitious shoots more than 1.0 cm in height were selected for rooting. On rooting medium supplemented with 0.1 mg l-1 indole-3-butyric acid, 1 mg l-1 BA, and 0.5 mg l-1 gibberellic acid, the highest rooting frequency of adventitious shoots was 46% in a culture period of 6 weeks. Established plants survived following transfer to soil at a frequency of 71%.