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A microplate culture system has been standardized for blastic transformation of lymphocytes, using various mitogens more or less specific to T lymphocytes (concanavalin A, phytohaemagglutinin, erythrogenic toxin) or B lymphocytes (lipopolysaccharides and their lipids A, pokeweed mitogen). The mitogen-stimulated lymphocyte activation was examined by the use of the nucleolar test determining the state of nucleolar RNA synthesis by morphologic criteria. In this introductory paper to a series of papers analyzing the specificity of action of the various chemical and biological immunosuppressive agents on the blastogenic responses of T and B lymphocytes, optimal concentrations of cells and blastogenic substances and other parameters were tested, and the kinetics of transformation was investigated in detail.
M Pospísil, and
P Dráber, and
J Hofman, and
K Nouza, and
J Klapácová
January 1974,
Proceedings of the Clinical Dialysis and Transplant Forum,
M Pospísil, and
P Dráber, and
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J Klapácová
April 1977,
Biological psychiatry,
M Pospísil, and
P Dráber, and
J Hofman, and
K Nouza, and
J Klapácová
October 1975,
Clinical and experimental immunology,
M Pospísil, and
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July 1977,
Immunology,
M Pospísil, and
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J Hofman, and
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February 1970,
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M Pospísil, and
P Dráber, and
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K Nouza, and
J Klapácová
March 1983,
Biochemical pharmacology,
M Pospísil, and
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K Nouza, and
J Klapácová
January 1994,
American journal of primatology,
M Pospísil, and
P Dráber, and
J Hofman, and
K Nouza, and
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M Pospísil, and
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J Hofman, and
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J Klapácová
July 1977,
Blood,
M Pospísil, and
P Dráber, and
J Hofman, and
K Nouza, and
J Klapácová
February 1975,
Immunology,
