Purification and characteristics of an aflatoxin B1 degradation enzyme isolated from Pseudomonas aeruginosa. 2019

Juanjuan Song, and Shujie Zhang, and Yanli Xie, and Qian Li
School of Food Science and Technology, Henan University of Technology, 100# Lianhua Street, High-tech Industrial Development Zone, Zhengzhou 450001, Henan, People's Republic of China.

Aflatoxin is a highly toxic mycotoxin produced by Aspergillus flavus and Aspergillusparasiticus fungus. Since it contaminates food and grain widely, it has seriously endanger the health of human beings and animals. Some microorganisms that exist in nature can degrade aflatoxin. In this paper, the biological AFB1 (aflatoxin B1)-degradation by Pseudomonas aeruginosa M19 was evaluated. We found that the culture supernatant of P. aeruginosa M19 added with proteinase K (Prok), SDS and heating significantly decreased degradation capacity. Pseudomonas AFB1-degrading enzyme (PADE) was purified from P. aeruginosa M19 by a three-step procedure including ammonium sulfate fractional precipitation and ion exchange chromatography and gel filtration chromatography. PADE was purified 175-fold with a recovery of 35%, and an ultimate specific activity of 6.1 × 104 U/mg was achieved. The molecular weight of PADE estimated by SDS-PAGE is about 48 kDa. PADE displayed the highest degradation activity for AFB1 at 65°C and pH 6.0. Cu2+ and Fe3+ strongly enhanced the activity, whereas Ca2+ and Zn2+ strongly inhibited the activity. These findings indicate that PADE from P. aeruginosa M19 is a promising candidate for AFB1 biodegradation.

UI MeSH Term Description Entries
D008670 Metals Electropositive chemical elements characterized by ductility, malleability, luster, and conductance of heat and electricity. They can replace the hydrogen of an acid and form bases with hydroxyl radicals. (Grant & Hackh's Chemical Dictionary, 5th ed) Metal
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D011550 Pseudomonas aeruginosa A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection. Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D004795 Enzyme Stability The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat. Enzyme Stabilities,Stabilities, Enzyme,Stability, Enzyme
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D001673 Biodegradation, Environmental Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers. Bioremediation,Phytoremediation,Natural Attenuation, Pollution,Environmental Biodegradation,Pollution Natural Attenuation
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures
D016604 Aflatoxin B1 A potent hepatotoxic and hepatocarcinogenic mycotoxin produced by the Aspergillus flavus group of fungi. It is also mutagenic, teratogenic, and causes immunosuppression in animals. It is found as a contaminant in peanuts, cottonseed meal, corn, and other grains. The mycotoxin requires epoxidation to aflatoxin B1 2,3-oxide for activation. Microsomal monooxygenases biotransform the toxin to the less toxic metabolites aflatoxin M1 and Q1. Aflatoxin B(1),Aflatoxin B,Aflatoxin B1 Dihydrochloride, (6aR-cis)-Isomer,Aflatoxin B1, (6aR-cis)-Isomer, 14C-Labeled,Aflatoxin B1, (6aR-cis)-Isomer, 2H-Labeled,Aflatoxin B1, (6aR-cis)-Isomer, 3H-Labeled,Aflatoxin B1, cis(+,-)-Isomer,HSDB-3453,NSC-529592,HSDB 3453,HSDB3453,NSC 529592,NSC529592

Related Publications

Juanjuan Song, and Shujie Zhang, and Yanli Xie, and Qian Li
January 2019, Toxicon : official journal of the International Society on Toxinology,
Juanjuan Song, and Shujie Zhang, and Yanli Xie, and Qian Li
January 2011, Journal of applied microbiology,
Juanjuan Song, and Shujie Zhang, and Yanli Xie, and Qian Li
January 2003, Microbiological research,
Juanjuan Song, and Shujie Zhang, and Yanli Xie, and Qian Li
March 2023, Foods (Basel, Switzerland),
Juanjuan Song, and Shujie Zhang, and Yanli Xie, and Qian Li
May 2020, Toxicon : official journal of the International Society on Toxinology,
Juanjuan Song, and Shujie Zhang, and Yanli Xie, and Qian Li
November 1967, British journal of pharmacology and chemotherapy,
Juanjuan Song, and Shujie Zhang, and Yanli Xie, and Qian Li
October 2019, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology],
Juanjuan Song, and Shujie Zhang, and Yanli Xie, and Qian Li
June 1969, The Journal of antibiotics,
Juanjuan Song, and Shujie Zhang, and Yanli Xie, and Qian Li
January 1972, Japanese journal of microbiology,
Juanjuan Song, and Shujie Zhang, and Yanli Xie, and Qian Li
March 1967, Journal of bacteriology,
Copied contents to your clipboard!