-transparent.png){kind=logo}
The cellular concentration of AdoMet is normally very much higher than that of dcAdoMet, the aminopropyl group donor in polyamine synthesis. However, when cells are depleted of their putrescine and spermidine, i.e. the aminopropyl group acceptors, the dcAdoMet concentration increases dramatically, to a level that may greatly exceed that of AdoMet. Using a highly purified DNA methyltransferase and its preferred substrates, a defined hemimethylated duplex oligodeoxynucleotide or poly(dI-dC)-poly(dI-dC), we demonstrate that dcAdoMet is a poor methyl group donor, and that it starts to inhibit DNA methylation when its concentration exceeds that of AdoMet. At a dcAdoMet/AdoMet ratio of 5:1 there is very little methyl transfer. This study suggests that the antiproliferative and differentiative effects brought about by inhibitors of polyamine synthesis may be partly attributable to dcAdoMet-mediated inhibition of DNA methylation.
O Heby, and
L Persson, and
S S Smith
January 1994,
Critical reviews in microbiology,
O Heby, and
L Persson, and
S S Smith
April 2007,
Biochemical Society transactions,
O Heby, and
L Persson, and
S S Smith
November 2021,
Cells,
O Heby, and
L Persson, and
S S Smith
February 1979,
Biochemical and biophysical research communications,
O Heby, and
L Persson, and
S S Smith
January 2020,
Critical reviews in immunology,
O Heby, and
L Persson, and
S S Smith
March 1989,
Environmental health perspectives,
O Heby, and
L Persson, and
S S Smith
October 1995,
The International journal of developmental biology,
O Heby, and
L Persson, and
S S Smith
May 1993,
Leukemia,
O Heby, and
L Persson, and
S S Smith
August 2021,
Nature cell biology,
O Heby, and
L Persson, and
S S Smith
March 1985,
Tsitologiia,
