Dengue is one of the most rapidly spreading infectious diseases prevalent throughout the tropical and sub-tropical regions of the world. In absence of specific medications and vaccines, the sole method of disease prevention relies on vector control mainly using insecticides. But with the advent of Insecticide resistance, worldwide vector control programs are facing failure. In this study, eleven different Ae. albopictus population from sub-Himalayan districts of West Bengal, India were investigated as per WHO protocols to find out the current status of insecticide susceptibility against DDT, permethrin and propoxur. Also the role of three insecticide detoxifying enzymes underlying observed resistance was investigated through quantitative and synergistic assays to unveil the mechanism of insecticide resistance. It was found that majority of studied populations were resistant to 4% DDT. Two populations, namely Alipurduar (APD) and Jalpaiguri (JPG) were severely resistant to 0.75% permethrin, whereas only JPG population was found to exhibit severe resistance against 0.1% propoxur. Moreover, the involvement of detoxifying enzymes was also noted in conferring resistance against DDT and Permethrin. This study indicates the inefficacy of DDT in controlling Ae. albopictus populations in the study region. This study may help in implementation of an efficient vector control and insecticide resistance management strategies.