Heat Resistant Psychrotrophic Bacteria in Raw Milk and Their Growth at 7 C. 1978

E M Mikolajcik, and N T Simon
Department of Food Science and Nutrition The Ohio State University, Columbus, Ohio 43210.

Microbiological data are presented for 109 raw milk samples, and for the same samples after heat treatment at 80 C for 12 min, and upon subsequent storage at 7 C for 7, 14, and 28 days. The Standard Plate Count of the raw milk averaged 110,000/ml with 65% of these organisms being penicillin-resistant. Immediately after heat treatment, 87% of the samples had psychrotrophic spore counts< 10/ml. After 14 and 28 days of storage, 50 and 83% of the samples had psychrotrophic counts ≥ 100,000/ml. It was concluded that growth of heat-resistant psychrotrophic organisms may cause spoilage of heated milk. No relationship could be demonstrated between gram-negative counts of raw milk, or initial mesophilic spore counts of heated milk, and bacterial numbers in heated, stored milk.

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