Biomaterial Database

Microbial synergistic interactions for reductive dechlorination of polychlorinated biphenyls. 2019

Shanquan Wang, and Chen Chen, and Siyan Zhao, and Jianzhong He

Department of Civil and Environmental Engineering, National University of Singapore, 117576, Singapore. Electronic address: wangshanquan@mail.sysu.edu.cn.

Dehalococcoides usually work closely with other beneficial microorganisms for removal of halogenated organic compounds at contaminated sites. Traditional microbial cultivation is necessary but not enough to gain insights into key microbial populations and their interactions in complex communities. In this study, we cultivated and characterized two D. mccartyi strains (CG3 and SG1), and further revealed interspecies synergistic interactions in PCB-dechlorinating microbial communities via metagenomic analysis. Strain CG3 and SG1 originated from distinct geographic sites employ reductive dehalogenase CG3-RD11 (PcbA1-like) and SG1-RD28 (PcbA4/5-like), respectively, to catalyze chlorine-removal from PCBs. In their parent mixed cultures CG-3 and SG-1, as well as in previously enriched PCB-dechlorinating cultures CG-1, CG-4 and CG-5, Methanosarcina and Desulfovibrio were found as major non-dechlorinating populations which may play roles in mediating acetate- and H2-sources for D. mccartyi. They together form a stable microbial community for interspecies carbon- and electron-transfers to facilitate organohalide respiration of D. mccartyi, being confirmed in a synthetic microbial community consisting of the Dehalococcoides, Methanosarcina and Desulfovibrio. The results provide insights into which and how other microorganisms support D. mccartyi to dechlorinate PCBs, and suggest that Methanosarcina may play a larger role in PCB-dechlorinating communities than currently appreciated.

UI	MeSH Term	Description	Entries
D011078	Polychlorinated Biphenyls	Industrial products consisting of a mixture of chlorinated biphenyl congeners and isomers. These compounds are highly lipophilic and tend to accumulate in fat stores of animals. Many of these compounds are considered toxic and potential environmental pollutants.	PCBs,Polychlorinated Biphenyl,Polychlorobiphenyl Compounds,Biphenyl, Polychlorinated,Biphenyls, Polychlorinated,Compounds, Polychlorobiphenyl
D002713	Chlorine	An element with atomic symbol Cl, atomic number 17, and atomic weight 35, and member of the halogen family.	Chlorine Gas,Chlorine-35,Cl2 Gas,Chlorine 35,Gas, Chlorine,Gas, Cl2
D001419	Bacteria	One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.	Eubacteria
D056265	Microbial Interactions	The inter- and intra-relationships between various microorganisms. This can include both positive (like SYMBIOSIS) and negative (like ANTIBIOSIS) interactions. Examples include virus - bacteria and bacteria - bacteria.	Competitive Behavior, Microbial,Cooperative Behavior, Microbial,Behavior, Microbial Competitive,Behavior, Microbial Cooperative,Behaviors, Microbial Competitive,Behaviors, Microbial Cooperative,Competitive Behaviors, Microbial,Cooperative Behaviors, Microbial,Interaction, Microbial,Interactions, Microbial,Microbial Competitive Behavior,Microbial Competitive Behaviors,Microbial Cooperative Behavior,Microbial Cooperative Behaviors,Microbial Interaction