Biomaterial Database

Distinguishing Colony Opacity Variants and Measuring Opacity Variation in Acinetobacter baumannii. 2019

Sarah E Anderson, and Philip N Rather

Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA.

Many strains of Acinetobacter baumannii, including the highly virulent strain AB5075, undergo a high-frequency switch that results in two cell types that are distinguished by their opaque or translucent colony opacities when viewed by oblique lighting. Opaque (VIR-O) and translucent (AV-T) colonies exhibit multiple phenotypic differences, including virulence. Here we describe how to distinguish between VIR-O and AV-T colony variants and how to generate highly pure stocks of each variant. We also describe methods for measuring opacity switching frequencies of cells grown on agar plates and in liquid cultures.

UI	MeSH Term	Description	Entries
D010641	Phenotype	The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.	Phenotypes
D000151	Acinetobacter Infections	Infections with bacteria of the genus ACINETOBACTER.	Mimae Infections,Infections, Acinetobacter,Infections, Mimae,Acinetobacter Infection,Infection, Acinetobacter,Infection, Mimae,Mimae Infection
D015373	Bacterial Typing Techniques	Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.	Bacteriocin Typing,Biotyping, Bacterial,Typing, Bacterial,Bacterial Biotyping,Bacterial Typing,Bacterial Typing Technic,Bacterial Typing Technics,Bacterial Typing Technique,Technic, Bacterial Typing,Technics, Bacterial Typing,Technique, Bacterial Typing,Techniques, Bacterial Typing,Typing Technic, Bacterial,Typing Technics, Bacterial,Typing Technique, Bacterial,Typing Techniques, Bacterial,Typing, Bacteriocin
D058491	Bacterial Load	Measurable quantity of bacteria in an object, organism, or organism compartment.	Bacterial Count,Bacterial Counts,Count, Bacterial,Counts, Bacterial,Bacterial Loads,Load, Bacterial,Loads, Bacterial
D040981	Acinetobacter baumannii	A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.	Bacterium anitratum