Knowing the virulence composition of Phytophthora sojae is important for the management of Phytophthora root and stem rot of soybean. Plant samples were collected in Michigan from diseased plants in soybean fields with Phytophthora root and stem rot symptoms. Eighty-seven isolates of P. sojae were evaluated for virulence using 13 differential soybean cultivars. Rps 3b, 3a, 1b, 1k, and 6 were resistant to 81, 77, 74, 69, and 66% of the isolates, respectively, while Rps 1a and 7 were resistant to only 12 to 13% of the isolates. Races 2, 25, 41, and 44 of P. sojae were identified among the isolates and reported for the first time in Michigan. Virulence formulae of 69 isolates did not match those of currently known races of P. sojae. Nine isolates were considered nonpathogenic. Incorporating Rps genes 1b, 1k, 3a, 3b, and 6 in soybean cultivars with good field tolerance, in conjunction with other control measures, should offer improved protection of soybeans from Phytophthora root and stem rot in Michigan.