Biomaterial Database

Regulation by nitric oxide on mitochondrial permeability transition of peaches during storage. 2019

Dandan Huang, and Shan Hu, and Shuhua Zhu, and Jianrong Feng

College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, 271018, China.

Mitochondrial membrane permeability transition pores (MPTP) play important roles in mitochondrial function. There are many chemicals in the mitochondria that can act as signal molecules to affect the membrane permeability of mitochondria and mediate to release various enzymes. As a signaling molecule, nitric oxide (NO) is a key player in fruit growth and development. However, the specific mechanism through NO regulates MPTP, and how exogenous NO prolongs fruit storage time are both unclear. In this study, Feicheng peaches were treated with different concentrations of exogenous NO (5, 15 and 30 μmol L-1) and c-PTIO to determine the changes in mitochondrial membrane potential (MMP), hexokinase II activity, the contents of cytochrome C and Ca2+ in mitochondria, as well as the effects of voltage-dependent anion channels (VDAC) and phosphate carrier (PiC) proteins on MPTP during storage. The results showed that NO could form a 1:1 complex either with VDAC or PiC, which proved that NO could react with the protein of PiC or VDAC. Treatment with 15 μmol L-1 NO maintained stable mitochondrial Ca2+ content, and high potential and permeability of the mitochondrial membrane, while decreased cytochrome C content and increased hexokinase activity. When NO was removed, the opposite result appeared. These results indicated that exogenous NO could stabilize MMP and participate in MPTP regulation of peaches during storage.

UI	MeSH Term	Description	Entries
D008928	Mitochondria	Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)	Mitochondrial Contraction,Mitochondrion,Contraction, Mitochondrial,Contractions, Mitochondrial,Mitochondrial Contractions
D009569	Nitric Oxide	A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.	Endogenous Nitrate Vasodilator,Mononitrogen Monoxide,Nitric Oxide, Endothelium-Derived,Nitrogen Monoxide,Endothelium-Derived Nitric Oxide,Monoxide, Mononitrogen,Monoxide, Nitrogen,Nitrate Vasodilator, Endogenous,Nitric Oxide, Endothelium Derived,Oxide, Nitric,Vasodilator, Endogenous Nitrate
D010539	Permeability	Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.	Permeabilities
D010940	Plant Proteins	Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which PLANT PROTEINS, DIETARY is available.	Plant Protein,Protein, Plant,Proteins, Plant
D005638	Fruit	The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds.	Berries,Legume Pod,Plant Aril,Plant Capsule,Aril, Plant,Arils, Plant,Berry,Capsule, Plant,Capsules, Plant,Fruits,Legume Pods,Plant Arils,Plant Capsules,Pod, Legume,Pods, Legume
D000068239	Prunus persica	A plant species in the family ROSACEAE. It is a tree that produces peach fruit.	Peach,Peach Tree,Peaches,Peach Trees,Tree, Peach,Trees, Peach
D000083162	Mitochondrial Permeability Transition Pore	A multiprotein inner mitochondrial complex which opens only under certain pathological conditions (e.g., OXIDATIVE STRESS) uncoupling the membrane leading to APOPTOSIS and MITOCHONDRIAL TRANSMEMBRANE PERMEABILITY-DRIVEN NECROSIS particularly in CARDIOMYOCYTES during MYOCARDIAL REPERFUSION INJURY.	Mitochondrial Megachannel,Mitochondrial Permeability Transition Pore (mPTP),mPTP Protein
D024101	Mitochondrial Proteins	Proteins encoded by the mitochondrial genome or proteins encoded by the nuclear genome that are imported to and resident in the MITOCHONDRIA.	Proteins, Mitochondrial,Mitochondrial Protein,Protein, Mitochondrial
D033681	Mitochondrial Membrane Transport Proteins	Proteins involved in the transport of specific substances across the membranes of the MITOCHONDRIA.	Membrane Transport Proteins, Mitochondrial,Mitochondrial Carrier Proteins,Mitochondrial Carriers,Mitochondrial Transport Proteins,Carrier Proteins, Mitochondrial,Transport Proteins, Mitochondrial