Development of Muscina stabulans at constant temperatures with implications for minimum postmortem interval estimation. 2019

Yu Wang, and Guoliang Hu, and Yingna Zhang, and Man Wang, and Jens Amendt, and Jiangfeng Wang
Department of Forensic Medicine, Soochow University, Ganjiang East Road, Suzhou, China.

Muscina stabulans (Fallén 1817) is a disease vector and a species of forensic value, particularly in the case of buried bodies. In this study, M. stabulans was reared under a total of seven constant temperatures at 16-34 °C, and the developmental duration, accumulated degree days, and larval body length were studied. From the results, three developmental models were established for estimating the minimum postmortem interval (PMImin), including isomorphen diagram, isomegalen diagram, and thermal summation model. Equations used to estimate the age of M. stabulans larvae were obtained by regression analysis using larval body length as the independent variable and time after hatching as the dependent variable, or vice versa. M. stabulans completed development at 16-31 °C, while it did not complete development at 34 °C, where puparia failed to transform into adults. The average developmental duration from oviposition to eclosion at 16, 19, 22, 25, 28 and 31 °C were 929.7 ± 31.7, 693.5 ± 4.8, 515.3 ± 4.6, 392.5 ± 9.0, 334.0 ± 7.8, 331.5 ± 4.71 h, respectively. We used a linear thermal summation model and estimated that the lower developmental threshold temperature was 9.87 ± 0.33 °C and the thermal summation constant was 253.16 ± 11.32 degree days. Lower developmental thresholds, intrinsic optimum temperature and upper lethal developmental threshold temperature were estimated by a nonlinear model to be 9.56, 21.63 and 32.85 °C, respectively. Our study provides data for the use of M. stabulans for PMImin estimation.

UI MeSH Term Description Entries
D007814 Larva Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals. Maggots,Tadpoles,Larvae,Maggot,Tadpole
D009117 Muscidae A family of the order DIPTERA with over 700 species. Important species that may be mechanical vectors of disease include Musca domesticus (HOUSEFLIES), Musca autumnalis (face fly), Stomoxys calcitrans (stable fly), Haematobia irritans (horn fly) and Fannia spp. Face Fly,Fannia,Haematobia irritans,Horn Fly,Musca autumnalis,Stable Fly,Stomoxys calcitrans,Face Flies,Fannias,Flies, Face,Flies, Horn,Flies, Stable,Fly, Face,Fly, Horn,Fly, Stable,Haematobia irritan,Horn Flies,Musca autumnali,Stable Flies,Stomoxys calcitran,autumnali, Musca,calcitran, Stomoxys,irritan, Haematobia
D010058 Oviposition The process of laying or shedding fully developed eggs (OVA) from the female body. The term is usually used for certain INSECTS or FISHES with an organ called ovipositor where eggs are stored or deposited before expulsion from the body. Larviposition,Larvipositions,Ovipositions
D011180 Postmortem Changes Physiological changes that occur in bodies after death. Adipocere,Algor Mortis,Cruor,Livor Mortis,Change, Postmortem,Changes, Postmortem,Postmortem Change
D011679 Pupa An inactive stage between the larval and adult stages in the life cycle of INSECTA. Chrysalis,Pupae
D004772 Entomology A discipline or occupation concerned with the study of INSECTS, including the biology and the control of insects.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013048 Specimen Handling Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation. Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures

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