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Identification of the microbial diversity after fecal microbiota transplantation therapy for chronic intractable constipation using 16s rRNA amplicon sequencing. 2019

Tadashi Ohara
Department of Intestinal Biosciences and Medicine, Fukushima Medical University, Fukushima, Japan.

Fecal microbiota transplantation (FMT) is an effective therapeutic approach for the treatment of functional gastrointestinal disease by restoring gut microbiota; however, there is a lack of sufficient understanding regarding which microbial populations successfully colonize the recipient gut. This study characterized microbial composition and diversity in patients diagnosed with chronic constipation at 1 month and 1 year after FMT. We explored the microbial diversity of pre- and posttransplant stool specimens from patients using 16S rRNA gene sequencing, followed by functional analysis. The results identified 22 species of microorganisms colonized in the recipients from the donors at 1 month after FMT. One-year follow-up of the patient identified the colonization of 18 species of microorganisms, resulting in identification of species in significant abundance, including Bacteroides fragilis and Hungatella hathewayi in the recipient at 1 month after FMT and Dialister succinatiphilus, Coprococcus catus, and Sutterella stercoricanis at 1 year after FMT. The majority of the colonized species belong to the phylum Firmicutes and carry genes related to polysaccharide metabolism and that enhance the energy-harvesting efficiency of the host. These results suggest that FMT is effective for the treatment of chronic constipation through the restoration and colonization of donor microbiota in the recipient gut up to 1 year after FMT.

UI MeSH Term Description Entries
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D002908 Chronic Disease Diseases which have one or more of the following characteristics: they are permanent, leave residual disability, are caused by nonreversible pathological alteration, require special training of the patient for rehabilitation, or may be expected to require a long period of supervision, observation, or care (Dictionary of Health Services Management, 2d ed). For epidemiological studies chronic disease often includes HEART DISEASES; STROKE; CANCER; and diabetes (DIABETES MELLITUS, TYPE 2). Chronic Condition,Chronic Illness,Chronically Ill,Chronic Conditions,Chronic Diseases,Chronic Illnesses,Condition, Chronic,Disease, Chronic,Illness, Chronic
D003248 Constipation Infrequent or difficult evacuation of FECES. These symptoms are associated with a variety of causes, including low DIETARY FIBER intake, emotional or nervous disturbances, systemic and structural disorders, drug-induced aggravation, and infections. Colonic Inertia,Dyschezia
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000069467 Fecal Microbiota Transplantation Transfer of GASTROINTESTINAL MICROBIOTA from one individual to another by infusion of donor FECES to the upper or lower GASTROINTESTINAL TRACT of the recipient. Fecal Microbiome Transplantation,Fecal Microbiota Transfer,Fecal Microbiota Transplant,Intestinal Microbiome Transfer,Intestinal Microbiome Transplant,Intestinal Microbiome Transplantation,Intestinal Microbiota Transplant,Intestinal Microbiota Transplantation,Donor Feces Infusion,Fecal Transplant,Fecal Transplantation,Intestinal Microbiota Transfer,Donor Feces Infusions,Fecal Microbiome Transplantations,Fecal Microbiota Transfers,Fecal Microbiota Transplantations,Fecal Microbiota Transplants,Fecal Transplantations,Fecal Transplants,Feces Infusion, Donor,Feces Infusions, Donor,Infusion, Donor Feces,Infusions, Donor Feces,Intestinal Microbiome Transfers,Intestinal Microbiome Transplantations,Intestinal Microbiome Transplants,Intestinal Microbiota Transfers,Intestinal Microbiota Transplantations,Intestinal Microbiota Transplants,Microbiome Transfer, Intestinal,Microbiome Transfers, Intestinal,Microbiome Transplant, Intestinal,Microbiome Transplantation, Fecal,Microbiome Transplantation, Intestinal,Microbiome Transplantations, Fecal,Microbiome Transplantations, Intestinal,Microbiome Transplants, Intestinal,Microbiota Transfer, Fecal,Microbiota Transfer, Intestinal,Microbiota Transfers, Fecal,Microbiota Transfers, Intestinal,Microbiota Transplant, Fecal,Microbiota Transplant, Intestinal,Microbiota Transplantation, Fecal,Microbiota Transplantation, Intestinal,Microbiota Transplantations, Fecal,Microbiota Transplantations, Intestinal,Microbiota Transplants, Fecal,Microbiota Transplants, Intestinal,Transfer, Fecal Microbiota,Transfer, Intestinal Microbiome,Transfer, Intestinal Microbiota,Transfers, Fecal Microbiota,Transfers, Intestinal Microbiome,Transfers, Intestinal Microbiota,Transplant, Fecal,Transplant, Fecal Microbiota,Transplant, Intestinal Microbiome,Transplant, Intestinal Microbiota,Transplantation, Fecal,Transplantation, Fecal Microbiome,Transplantation, Fecal Microbiota,Transplantation, Intestinal Microbiome,Transplantation, Intestinal Microbiota,Transplantations, Fecal,Transplantations, Fecal Microbiome,Transplantations, Fecal Microbiota,Transplantations, Intestinal Microbiome,Transplantations, Intestinal Microbiota,Transplants, Fecal,Transplants, Fecal Microbiota,Transplants, Intestinal Microbiome,Transplants, Intestinal Microbiota
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D001419 Bacteria One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive. Eubacteria

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